Genotoxicity and the stability of N-nitrosomorpholine activity following UVA irradiation

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-12-02 DOI:10.1016/j.mrgentox.2023.503721

Haruna Mochizuki , Yukari Nagazawa , Sakae Arimoto-Kobayashi

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Abstract

This study investigated N-nitrosomorpholine (NMOR) genotoxicity following UVA irradiation without metabolic activation. Following UVA irradiation, the photo treated NMOR (irradiated NMOR) was directly mutagenic, without UVA or metabolic activation, in the Ames test. The activity was relatively stable, and approximately 79% of the activity remained after 10 days of storage at 37 °C, 4 °C, or −20 °C. Micronuclei (MN) formation was observed in HaCaT cells after treatment with irradiated NMOR without metabolic activation. The action spectrum of MN formation in response to NMOR irradiation followed the NMOR absorption curve. In vivo, MN formation was observed in the peripheral blood reticulocytes of mice injected with irradiated NMOR under the inhibition of cytochrome P450-mediated metabolism of NMOR. Volatile NMOR may attach to environmental materials and be irradiated with environmental UVA light. Photoactivated NMOR-attached air pollutants could float in the air and fall onto the human body, leading to genotoxicity induced by the irradiated NMOR.

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UVA辐照后n -亚硝基somorpholine活性的遗传毒性和稳定性

本研究研究了在无代谢激活的UVA照射下n -亚硝基somorpholine (NMOR)的遗传毒性。经过UVA照射后，经过光处理的NMOR(辐照后的NMOR)在Ames试验中直接致突变，没有UVA或代谢激活。活性相对稳定，在37°C、4°C或-20°C条件下保存10天后，约79%的活性仍保持不变。在没有代谢激活的情况下，辐照NMOR处理HaCaT细胞观察到微核(MN)的形成。MN在NMOR辐照下的作用谱符合NMOR吸收曲线。在体内，在细胞色素p450介导的NMOR代谢受到抑制的情况下，注射辐照NMOR的小鼠外周血网状细胞中观察到MN的形成。挥发性NMOR可以附着在环境材料上，并被环境UVA光照射。光活化的NMOR附着的空气污染物可以在空气中漂浮并落到人体上，导致NMOR辐照后引起的遗传毒性。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.