Genome editing in plants: a tool for precision breeding and functional genomics

Abstract

Genome or gene editing (GE) involves a repertoire of innovative molecular techniques that make use of sequence-specific nucleases (SSNs), for the precise modification of an organism's genome sequences. The CRISPR/Cas-based GE system, associated with Clustered Regularly Interspaced Short Palindromic Repeats, has emerged as a potent addition to the expanding genomics toolkit. It enables precise mutagenesis, gene knockouts, multiplex gene editing, and the manipulation of gene expression in plants. Undoubtedly, the application of CRISPR/Cas-based GE in plants has brought about a revolution in basic research, aiding in our understanding of gene functions and significantly advancing applied crop research. This, in turn, underscores its immense potential for crop improvement. Against this backdrop, the current Special Issue on "Genome Editing in Plants: A Tool for Precision Breeding and Functional Genomics" represents a timely effort to assemble a group of leading experts in the field of plant genome editing. This compilation includes a commentary article, two original research papers, and eleven review articles and is expected to bring about substantial progress in the field of plant science, particularly in the domain of genome editing.