Behavior of breast cancer cells under oxygen concentration gradients in a microfluidic device.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference Pub Date : 2023-07-01 DOI:10.1109/EMBC40787.2023.10340327

Satoshi Aratake, Naoto Kawahara, Kenichi Funamoto

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Abstract

The tumor microenvironment (TME) is known as a chronic hypoxic environment, with spatiotemporal variation in oxygen concentration depending on the distance from blood vessels and the blood supply. In our previous studies, cancer cell behavior was observed under hypoxic conditions with spatial variation of oxygen concentration (oxygen concentration gradients); however, that under oxygen concentration gradients at low oxygen levels found in the TME has not been studied. In this study, we investigated the behavior of breast cancer cells at various oxygen concentration gradients, generated using a microfluidic device with oxygen concentration controllability. The results showed that cell distribution was altered in response to oxygen concentration, and tended to increase in a specific region at around 5% O₂. Evaluation of changes in cell numbers due to proliferation, migration, and cell death indicated that proliferation strongly affected cell distribution.

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微流控装置中乳腺癌细胞在氧气浓度梯度下的行为。

众所周知，肿瘤微环境（TME）是一种慢性缺氧环境，氧浓度的时空变化取决于与血管的距离和血液供应。在我们之前的研究中，我们观察到了癌细胞在氧浓度空间变化（氧浓度梯度）的缺氧条件下的行为，但对 TME 中低氧浓度梯度下的癌细胞行为尚未进行研究。在本研究中，我们利用一个可控氧浓度的微流控装置，研究了乳腺癌细胞在不同氧浓度梯度下的行为。结果表明，细胞的分布随氧气浓度的变化而改变，在氧气浓度为 5%左右的特定区域，细胞的分布有增加的趋势。对增殖、迁移和细胞死亡导致的细胞数量变化的评估表明，增殖对细胞分布有很大影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference

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0.80

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