Characterization of the Chemical Constituents in Radix gentianae by Ultra-High Performance Liquid Chromatography Coupled with Quadrupole Time-of-Flight Mass Spectrometry

IF 1.3 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of chromatographic science Pub Date : 2023-12-20 DOI:10.1093/chromsci/bmad091

Xu Pan, Jiao Guan, Yue Cui, Lele Li, Xiaoying Lin, Bo Feng, Heyun Zhu

引用次数: 0

Abstract

Radix gentianae (RG) is a traditional Chinese medicine used for the treatment of acute and chronic hepatitis in clinic. However, the chemical profile of RG is still unconfirmed, which hindered the progress of pharmacological study and clinical application. In this study, ultra-high performance liquid chromatography together with quadrupole time-of-flight mass spectrometry techniques were employed to separate and characterize the chemical constituents in RG. Under the optimized conditions, a total of 60 compounds were rapidly identified or tentatively characterized. Results indicated that iridoid glucosides, flavonoids, organic acids, amino acids, saccharides and nucleosides were major constituents in RG. It is concluded the established method can help to clarify the substance basis and provide useful information for ascertaining the bioactive constituents and action mechanism of RG.

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超高效液相色谱-四极杆飞行时间质谱法表征龙胆草中的化学成分

龙胆草（RG）是一种传统中药，临床上用于治疗急慢性肝炎。然而，龙胆草的化学成分尚未得到证实，这阻碍了其药理研究和临床应用的进展。本研究采用超高效液相色谱法和四极杆飞行时间质谱法对 RG 中的化学成分进行了分离和表征。在优化的条件下，共对 60 种化合物进行了快速鉴定或初步定性。结果表明，鸢尾苷、黄酮、有机酸、氨基酸、糖类和核苷是 RG 中的主要成分。结论是所建立的方法有助于阐明 RG 的物质基础，并为确定 RG 的生物活性成分和作用机制提供有用信息。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.