{"title":"Hairpin extension a general method for the improvement of sensitivity of oligonucleotide probes","authors":"K.S. Sriprakash , J. Hartas","doi":"10.1016/0735-0651(89)90023-X","DOIUrl":null,"url":null,"abstract":"<div><p>A general and sensitive detection method of target DNA is described. The system is based on an oligonucleotide probe labeled to high specific activity. This involves a novel oligonucleotide design incorporating at the 3′ end a hairpin structure, allowing extension by polymerase reaction.</p></div>","PeriodicalId":77714,"journal":{"name":"Gene analysis techniques","volume":"6 2","pages":"Pages 29-32"},"PeriodicalIF":0.0000,"publicationDate":"1989-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0735-0651(89)90023-X","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gene analysis techniques","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/073506518990023X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
A general and sensitive detection method of target DNA is described. The system is based on an oligonucleotide probe labeled to high specific activity. This involves a novel oligonucleotide design incorporating at the 3′ end a hairpin structure, allowing extension by polymerase reaction.
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