The effect of mesenchymal stem cells from the adipose tissue and bone marrow of goats on the quality of their sperm cells

Abstract

The purpose of this research is to study the  influence  of  mesenchymal  stem  cells from  adipose  tissue  and  bone  marrow  of goats  on  the  quality  parameters  of  their sperm. A complex evaluation of sperm quality  (volume,  concentration,  morphology, motility) of Saanen and Czech goats (n=10) at the age of 1-2.5 years was carried out according  to  generally  accepted  methods  and protocols.  Adipose  tissue  (AT)  and  bone marrow  (BM)  were  obtained  after  the slaughter of goats (n=6) on a peasant farm in the Volkhov district of the Leningrad region. After processing the obtained samples, mesenchymal stem cells obtained from adipose tissue and bone marrow of goats were isolated,  accumulated  and  cryopreserved.  Three groups of samples of 10 each were formed: control (spermatozoa 7x1010 cells/ml + PBS buffer 100 μl, pH – 7.4), first experimental (spermatozoa  7x1010  cells/ml  +  MSCs 2x1010 cells/ml, pH – 7.2), the second was experimental (sperm 7x1010 cells/ml + BM MSCs 2x108 cells/ml, pH – 7.2) and a four-stage assessment of sperm quality was carried out: after incubation of sperm with mesenchymal  stem  cells  at  a  temperature  of 38℃ for 0, 1, 2 and 3 hours. Based on the results obtained, the working protocol for the use of mesenchymal stem cells from adipose tissue  and  bone  marrow  for  the  sperm  of breeding goats is the concentration of MSCs -  2x108  cells/ml  and  the  concentration  of goat  spermatozoa -  7x1010  cells/ml.  The studies conducted have established the positive effect of mesenchymal stem cells from the adipose tissue and bone marrow of goats on the quality indicators of their sperm. At the  same  time,  spermatozoa  remain  viable during three-hour incubation with a progression  of  movements  equal  to  51.5±8.46  - 51.8±5.2% and a number of morphologically normal  sperm  equal  to  50.40±2.21  - 51.10±3,  14%  (3  hours  after  incubation, MSCs from adipose tissue and bone marrow, respectively).