Rethinking c-Fos for understanding drug action in the brain.

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of biochemistry Pub Date : 2024-03-25 DOI:10.1093/jb/mvad110
Katsuyasu Sakurai
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Abstract

Understanding the mechanisms of drug action in the brain, from the genetic to the neural circuit level, is crucial for the development of new agents that act upon the central nervous system. Determining the brain regions and neurons affected by a drug is essential for revealing its mechanism of action in the brain. c-Fos, a marker of neuronal activation, has been widely used to detect neurons activated by stimuli with high spatial resolution. In this review, the use of c-Fos for the visualization and manipulation of activated neurons is introduced. I also explain that a higher temporal resolution can be achieved by changing the staining method for visualization of c-Fos. Moreover, a new method that allows labeling and manipulating commonly activated neurons using two different stimuli is proposed.

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重新思考 c-Fos,了解药物在大脑中的作用。
从基因到神经回路层面了解药物在大脑中的作用机制,对于开发作用于中枢神经系统的新药物至关重要。确定受药物影响的大脑区域和神经元对于揭示药物在大脑中的作用机制至关重要。c-Fos 是一种神经元激活标记物,已被广泛用于检测受刺激激活的神经元,具有很高的空间分辨率。在这篇综述中,我将介绍如何利用 c-Fos 对激活的神经元进行可视化和操作。我还解释了通过改变 c-Fos 可视化的染色方法可以实现更高的时间分辨率。此外,我还提出了一种新方法,可以使用两种不同的刺激标记和操纵常见的激活神经元。
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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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