Isolation of three different sizes of exosomes in an Asian population with different retinal diseases before and after treatment: preliminary results.

IF 4.2 4区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioengineered Pub Date : 2024-12-01 Epub Date: 2023-12-28 DOI:10.1080/21655979.2023.2297320
Sung-Yu Wu, Yu-Chien Hung, Chien-Chih Chou, Connie Chen, Chao-Min Cheng, Chihchen Chen, Jyh-Cheng Liou, Min-Yen Hsu
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Abstract

Exosomes are membranous structures measuring between 40-120 nm that are secreted by various cells of the human body into the body fluid system. Exosomes contain proteins, mRNA, miRNA, and signaling molecules, and physiologically they assist in the intercellular transport of proteins and RNA molecules. In this study, we used an immunoaffinity filter paper platform combined with scanning electron microscopy and microfluidic systems to detect the size of exosomes within the aqueous humor. Eight aqueous humor samples showed three distinct sizes of exosomes that were significantly different on scanning electron microscopy(P < 0.01). We further used nanoparticle tracking analysis to assess the size distribution of exosomes within the aqueous humor. We found significantly different distributions of exosomes between patients with three different ocular diseases and patients with normal cataracts as controls. An obvious peak of exomeres(size around 35 nm)was found in the patients with central retinal vein occlusion and vitreous hemorrhage. Flare-ups of large exosomes(size 90-120 nm)were found in the patients with the inflammatory ocular disease pars planitis. No obvious peaks in exomeres or large exosomes were found in the control group. There was a high association between the distribution of exosomes and the pathogenesis of ocular diseases. After intravitreal anti-vascular endothelial growth factor treatment, the aqueous humor from the patients with neovascular diseases showed a significant reduction in exosomes in nanoparticle tracking analysis. These findings suggest that at least three distinct sizes of exosomes exist in the aqueous humor:(1)exomeres:<35 nm;(2)small exosomes:60-80 nm; and (3)large exosomes:90-120 nm. Different sizes of exosomes may have different implications in normal or diseased eyes.

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在治疗前后患有不同视网膜疾病的亚洲人群中分离出三种不同大小的外泌体:初步结果。
外泌体是由人体内各种细胞分泌到体液系统中的40-120纳米的膜状结构。外泌体含有蛋白质、mRNA、miRNA 和信号分子,在生理上有助于蛋白质和 RNA 分子的细胞间转运。在这项研究中,我们利用免疫亲和滤纸平台,结合扫描电子显微镜和微流体系统,检测了房水中外泌体的大小。八个房水样本显示了三种不同大小的外泌体,这些外泌体在扫描电子显微镜下有显著差异(P
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来源期刊
Bioengineered
Bioengineered BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
8.20
自引率
28.60%
发文量
1114
审稿时长
17 weeks
期刊介绍: Bioengineered provides a platform for publishing high quality research on any aspect of genetic engineering which involves the generation of recombinant strains (both prokaryote and eukaryote) for beneficial applications in food, medicine, industry, environment and bio-defense.
期刊最新文献
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