{"title":"In Situ Imaging of Virus-Infected Cells by Cryo-Electron Tomography: An Overview.","authors":"Swetha Vijayakrishnan","doi":"10.1007/978-3-031-40086-5_1","DOIUrl":null,"url":null,"abstract":"<p><p>Cryo-electron tomography (cryo-ET) has emerged as a powerful tool in structural biology to study viruses and is undergoing a resolution revolution. Enveloped viruses comprise several RNA and DNA pleomorphic viruses that are pathogens of clinical importance to humans and animals. Considerable efforts in cryogenic correlative light and electron microscopy (cryo-CLEM), cryogenic focused ion beam milling (cryo-FIB), and integrative structural techniques are helping to identify virus structures within cells leading to a rise of in situ discoveries shedding light on how viruses interact with their hosts during different stages of infection. This chapter reviews recent advances in the application of cryo-ET in imaging enveloped viruses and the structural and mechanistic insights revealed studying the viral infection cycle within their eukaryotic cellular hosts, with particular attention to viral entry, replication, assembly, and egress during infection.</p>","PeriodicalId":21991,"journal":{"name":"Sub-cellular biochemistry","volume":"106 ","pages":"3-36"},"PeriodicalIF":0.0000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sub-cellular biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/978-3-031-40086-5_1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0
Abstract
Cryo-electron tomography (cryo-ET) has emerged as a powerful tool in structural biology to study viruses and is undergoing a resolution revolution. Enveloped viruses comprise several RNA and DNA pleomorphic viruses that are pathogens of clinical importance to humans and animals. Considerable efforts in cryogenic correlative light and electron microscopy (cryo-CLEM), cryogenic focused ion beam milling (cryo-FIB), and integrative structural techniques are helping to identify virus structures within cells leading to a rise of in situ discoveries shedding light on how viruses interact with their hosts during different stages of infection. This chapter reviews recent advances in the application of cryo-ET in imaging enveloped viruses and the structural and mechanistic insights revealed studying the viral infection cycle within their eukaryotic cellular hosts, with particular attention to viral entry, replication, assembly, and egress during infection.
低温电子断层扫描（cryo-ET）已成为结构生物学研究病毒的有力工具，并正在经历一场分辨率革命。包膜病毒包括几种 RNA 和 DNA 多形病毒，它们是对人类和动物具有重要临床意义的病原体。低温关联光电子显微镜（cryo-CLEM）、低温聚焦离子束铣削（cryo-FIB）和综合结构技术的大量应用有助于确定细胞内的病毒结构，从而增加了原位发现的数量，揭示了病毒在感染的不同阶段如何与其宿主相互作用。本章回顾了冷冻电子束成像技术在包膜病毒成像中的最新应用进展，以及研究真核细胞宿主内病毒感染周期所揭示的结构和机理，尤其关注感染过程中病毒的进入、复制、组装和排出。
期刊介绍：
The book series SUBCELLULAR BIOCHEMISTRY is a renowned and well recognized forum for disseminating advances of emerging topics in Cell Biology and related subjects. All volumes are edited by established scientists and the individual chapters are written by experts on the relevant topic. The individual chapters of each volume are fully citable and indexed in Medline/Pubmed to ensure maximum visibility of the work.
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