Upconverting-photon quenching-mediated perforation influx as an intracellular delivery method using posAuNP@UCNPs nanocomposites for osteoarthritis treatment

IF 13.4 2区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY Nano Convergence Pub Date : 2024-01-03 DOI:10.1186/s40580-023-00409-y

Hye Jin Kim, Hui Bang Cho, Hye-Ryoung Kim, Sujeong Lee, Ji-in Park, Keun-Hong Park

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Abstract

Photoporation techniques based on plasmonic nanoparticles such as gold nanoparticles have been extensively studied for the intracellular delivery of substances via cell membrane disruption. However, the clinical application of AuNP is challenging due to its absorption in the 500 nm region of the light spectrum. To overcome this challenge, upconversion nanoparticles were employed to stimulate AuNP at NIR wavelengths. posAuNP@UCNPs nanocomposites were produced by coating 30 nm UCNPs on 80 nm AuNPs using DOPA-PEI, which were then irradiated with 980 nm NIR light to facilitate their intracellular delivery. TEM and DLS confirmed that posAuNP and UCNP combine to form nanocomposites. Additionally, multiphysics simulation was used to analyze the distribution of the posAuNP electric field based on morphological differences that change as the UCNP ratio increases. Next, effective LED irradiation conditions were established by applying upconverting-photon quenching-mediated perforation influx to C28/I2 cells as suspensions or spheroids. posAuNP@UCNP nanocomposites were confirmed to be effective for the delivery of baricitinib as a treatment for osteoarthritis in a three-dimensional osteoarthritis model. Finally, chondrocyte differentiation was induced through intracellular delivery of baricitinib using posAuNP@UCNPs. The findings suggest that posAuNP@UCNPs have great potential as a tool for non-invasive drug delivery via UCPPin.

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利用posAuNP@UCNPs纳米复合材料的上转换-光子淬灭介导的穿孔流入作为细胞内给药方法治疗骨关节炎

人们广泛研究了基于金纳米粒子等质子纳米粒子的光穿透技术，该技术可通过破坏细胞膜在细胞内输送物质。然而，由于金纳米粒子在光谱的 500 纳米区域有吸收，其临床应用具有挑战性。使用 DOPA-PEI 将 30 nm 的 UCNPs 涂覆在 80 nm 的 AuNPs 上，制成 posAuNP@UCNPs 纳米复合材料，然后用 980 nm 的近红外光照射，促进其细胞内递送。TEM 和 DLS 证实，posAuNP 和 UCNP 结合形成纳米复合材料。此外，还利用多物理场模拟分析了 posAuNP 电场的分布，这种分布基于形态差异，随着 UCNP 比例的增加而变化。接下来，通过将上转换光子淬灭介导的穿孔流入应用于 C28/I2 细胞悬浮液或球形细胞，建立了有效的 LED 照射条件。在三维骨关节炎模型中，posAuNP@UCNP 纳米复合材料被证实可有效递送巴利昔尼（baricitinib），用于治疗骨关节炎。最后，使用 posAuNP@UCNPs 通过细胞内递送巴利替尼诱导了软骨细胞分化。研究结果表明，posAuNP@UCNPs 作为一种通过 UCPPin 进行非侵入性给药的工具具有巨大潜力。

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来源期刊

Nano Convergence Engineering-General Engineering

CiteScore

15.90

自引率

2.60%

发文量

审稿时长

13 weeks

期刊介绍： Nano Convergence is an internationally recognized, peer-reviewed, and interdisciplinary journal designed to foster effective communication among scientists spanning diverse research areas closely aligned with nanoscience and nanotechnology. Dedicated to encouraging the convergence of technologies across the nano- to microscopic scale, the journal aims to unveil novel scientific domains and cultivate fresh research prospects. Operating on a single-blind peer-review system, Nano Convergence ensures transparency in the review process, with reviewers cognizant of authors' names and affiliations while maintaining anonymity in the feedback provided to authors.