{"title":"Preparation of [1′-13C]citric acid as a probe in a breath test to evaluate tricarboxylic acid cycle flux","authors":"Hidemichi Mitome, Mieko Takenishi, Kumiko Ono, Naoyuki Kawagoe, Tsunehiko Imai, Yosuke Sasaki, Yoshihisa Urita, Kazuki Akira","doi":"10.1002/jlcr.4083","DOIUrl":null,"url":null,"abstract":"<p>[1′-<sup>13</sup>C]Citric acid (<b>1</b>) was efficiently prepared from dimethyl 1,3-acetonedicarboxylate in two steps as a probe for a breath test. The synthetic method was selected because of the yield and reproducibility. Compound <b>1</b> was orally administrated to rats, and the time course of the increase of <sup>13</sup>CO<sub>2</sub>/<sup>12</sup>CO<sub>2</sub> ratios (<i>Δ</i><sup>13</sup>CO<sub>2</sub>) in their breath was successfully followed, indicating the metabolism of <b>1</b>. Thus, the <sup>13</sup>C-breath test using <b>1</b> is a promising method to evaluate tricarboxylic acid (TCA) cycle flux.</p>","PeriodicalId":16288,"journal":{"name":"Journal of labelled compounds & radiopharmaceuticals","volume":"67 3","pages":"86-90"},"PeriodicalIF":0.9000,"publicationDate":"2024-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of labelled compounds & radiopharmaceuticals","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jlcr.4083","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
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Abstract
[1′-13C]Citric acid (1) was efficiently prepared from dimethyl 1,3-acetonedicarboxylate in two steps as a probe for a breath test. The synthetic method was selected because of the yield and reproducibility. Compound 1 was orally administrated to rats, and the time course of the increase of 13CO2/12CO2 ratios (Δ13CO2) in their breath was successfully followed, indicating the metabolism of 1. Thus, the 13C-breath test using 1 is a promising method to evaluate tricarboxylic acid (TCA) cycle flux.
期刊介绍：
The Journal of Labelled Compounds and Radiopharmaceuticals publishes all aspects of research dealing with labeled compound preparation and applications of these compounds. This includes tracer methods used in medical, pharmacological, biological, biochemical and chemical research in vitro and in vivo.
The Journal of Labelled Compounds and Radiopharmaceuticals devotes particular attention to biomedical research, diagnostic and therapeutic applications of radiopharmaceuticals, covering all stages of development from basic metabolic research and technological development to preclinical and clinical studies based on physically and chemically well characterized molecular structures, coordination compounds and nano-particles.
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