Hemoparasites effect on interferon-gamma and miRNA 125b expression in Bubalus bubalis

Abstract

This study aimed to determine and evaluate the effect of the g4467 G>A SNP on the expression profile of IFN-γ and miRNA 125b in dairy buffaloes (Bubalus bubalis) with and without hemoparasites. Molecular diagnosis was performed by Polymerase Chain Reaction (PCR) on 145 buffaloes for Babesia spp., Trypanosoma vivax , and Anaplasma marginale . All buffaloes were investigated for the presence or absence of the polymorphism and genotyped using the restriction enzyme. Real-time PCR (RT-qPCR) quantified the expression of IFN-γ and miRNA 125b. All buffaloes were negative for Babesia spp. and A . marginale , and only 12 were positive for T. vivax . The genotypes GG, GA, and AA were found in proportions of 3.4%, 2.1%, and 94.5%, respectively. The A allele was the most frequent (95.5%). The SNP showed deviation from Hardy-Weinberg equilibrium (HWE) (P<0.05) and a deficit of heterozygotes with FIS 0.759. All animals of the found genotypes expressed both genes, except for GG positive for T. vivax . IFN-γ expression was higher for GA and GG negative (P<0.05) and AA positive. However, miRNA 125b expression was lower for AA and GA positive and higher for AA, GA, and GG negative. AA-positive buffaloes for T. vivax may exhibit susceptibility due to higher IFN-γ expression and lower miRNA 125b. GG and GA-negative buffaloes exhibited higher expression in both, suggesting they have greater resistance to positive ones.