Unraveling the Role of Apoptosis in the Antiproliferative Activity of β-Glucan on A549 Cells

Abstract

Previous studies have reported the anticancer properties of β-glucan on various cancer cells. The objective of this research was to investigate the involvement of apoptosis in the cytotoxic action of β-glucan on the A549 cells. The cytotoxic impact of this drug on A549 cells was examined by subjecting them to various quantities of the substance, and the XTT assay was utilized to determine cell survival. Flow cytometry was performed to investigate apoptosis. A statistically significant and dose-dependent cytotoxic impact on A549 cells was observed upon treatment with β-glucan. The calculated IC50 value of β-glucan for A549 cells after a 24-hour treatment period was discovered to be 82.16 μg/mL. Further investigations carried out using the IC50 dose of β-glucan revealed a significant increase in the late apoptotic cells percentage. The capacity of β-glucan to trigger apoptosis is thought to be the cause of its cytotoxic action on lung cancer. The revelation of this discovery emphasizes the promising possibilities of β-glucan as an effective therapeutic choice.