The protective and antiapoptotic effects of Hypericum triquetrifolium Turra against cyclophosphamide-induced lung injury in rats: in vitro evaluation

The European Research Journal Pub Date : 2023-12-22 DOI:10.18621/eurj.1373134

Songul Cetik Yildiz, C. Keskin, V. Şahintürk, Adnan Ayhanci

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Abstract

Objectives: Hypericum triquetrifolium Turra (HTT) has been traditionally used in medical treatments due to its sedative, antiseptic, antiinflammatory, and anthelmintic properties. The present study aims to investigate the lung-protective and antiapoptotic effects of HTT against cyclophosphamide (CP)-induced lung injury in rats. Methods: Thirty-five Sprague Dawley rats were categorized into 5 groups, each consisting of seven members. Phenolic acid and flavonoid contents of this plant were determined. The lung tissue samples cultivated from the rats were examined in histopathological and immunohistochemically for the apoptosis markers of Caspase-3, Bax, and Bcl-2. Results: Histopathological results indicated that structural defects, bleeding areas, and edema had occurred in the lungs of the CP-Alone Group. Besides, Caspase-3 and Bax positivity of the lung cells had also increased while Bcl-2 positivity had decreased. On the other hand, in the HTT+CP Group, HTT was shown to have reversed the aforementioned changes positively. Conclusion: Based on in vivo results, HTT could be a strong protective candidate for CP-induced lung injury and apoptosis

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三棱金丝桃对环磷酰胺诱导的大鼠肺损伤的保护和抗凋亡作用：体外评估

目的：三棱金丝桃（HTT）具有镇静、杀菌、消炎和驱虫的作用，历来被用于医疗。本研究旨在探讨 HTT 对环磷酰胺（CP）诱导的大鼠肺损伤的保护和抗凋亡作用。研究方法将 35 只 Sprague Dawley 大鼠分为 5 组，每组 7 只。测定该植物的酚酸和类黄酮含量。对大鼠肺组织样本进行组织病理学和免疫组织化学检查，以检测 Caspase-3、Bax 和 Bcl-2 等细胞凋亡标志物。结果组织病理学结果表明，CP-Alone 组大鼠的肺部出现了结构缺陷、出血区和水肿。此外，肺细胞中 Caspase-3 和 Bax 阳性率也有所升高，而 Bcl-2 阳性率则有所降低。另一方面，在 HTT+CP 组中，HTT 对上述变化有积极的逆转作用。结论根据体内实验结果，HTT 对 CP 引起的肺损伤和细胞凋亡具有很强的保护作用。

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The European Research Journal

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