Ovocidal activity of microbial agent for composting in vitro experiment against Toxocara cati eggs

E. O. Kachanova, E. S. Belomyttseva, R. T. Safiullin, E. V. Puzanova
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Abstract

The purpose of the research is to study ovocidal properties of a microbial agent for composting that has lactobacilli (Lactobacillus casei and L. plantarum) and microscopic fungi (Saccharomyces cerevisiae) present as active components for Toxocara cati eggs in vitro experiment.Materials and methods. The tested microbial agent is used for compost preparation and liquid household waste processing. The agent is a homogeneous yellow liquid with a bacterial biomass in titers of 106 -108 CFU/cm3 which is placed in a ten-liter canister. First, a culture of Toxocara cati eggs was prepared, then ovocidal properties of aqueous standard solutions of the test agent were studied in four concentrations, 1.0%; 5.0; 10.0 and 50.0% versus a 4.0% phenol solution in vitro experiment.Results and discussion. The ovocidal effect of the tested aqueous microbiological agent solutions in the above concentrations in vitro experiment on T. cati eggs was not detected. A 4.0% phenol solution used as a reference solution showed high efficacy.
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堆肥微生物制剂体外实验对弓形虫卵的杀卵活性
本研究的目的是在体外实验中研究一种堆肥用微生物制剂的杀卵特性,该制剂以乳酸杆菌(Lactobacillus casei和L. plantarum)和微小真菌(Saccharomyces cerevisiae)为活性成分,用于杀灭Toxocara cati卵。测试的微生物制剂用于堆肥制备和液体生活垃圾处理。药剂是一种均匀的黄色液体,细菌生物量滴度为 106 -108 CFU/cm3,放置在一个 10 升的罐中。首先,制备了一种猫弓形虫卵培养物,然后在体外实验中研究了四种浓度(1.0%、5.0%、10.0%和 50.0%)的试验药剂水标准溶液与 4.0%苯酚溶液的杀卵特性。在体外实验中,未发现上述浓度的微生物剂水溶液对 T. cati 卵有杀卵作用。作为参考溶液的 4.0% 苯酚溶液显示出很高的效力。
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