Effect of Alcohol toxicity on estrous cycle, and in the uterus of Wistar rats

Abstract

In investigating the effect of Alcohol on the estrous cycle and uterine microstructure, hormonal and anti-oxidant concentration, twenty; adult female Wistar rats weighing 95 to 155 g were used. The animals were grouped into four groups of five rats each; group 1 is the control; while the animals in group 2-4 received 0.13 mL/kg, 0.67 mL/kg and 1.34 mL/kg of Alcohol. The administration was given with an orogastric tube orally for 28 days, weighed and sacrifice on the 29th day, using ketamine injection intraperitoneally. Blood, Uterus were harvested for hormonal and biochemical assays. The Uterus were processed using Hematoxylin and Eosin staining techniques and Masson trichrome stain for cyto-architectural assessment. The estrous cycle for the animals in group 1 had a regular cycle, Alcohol groups had irregular cycle, an increase in body weight of the animals in all the groups were recorded. Sections of Tissue showed hypertrophy of uterine glands in the high dose of alcohol group and Thickening; of epithelial lining for animals in group three. Alcohol groups had decreased staining intensity for the collagen fibers in the uterus. Catalase, Sodium dismutase activities decreased in group three and group four. Hormonal; concentrations decreased in alcohol groups with a statistical significance value of p < 0.05. Alcohol; can cause irregular estrous cycle, enlargement of the endometrial gland with symptoms such as menorrhagia, dysmenorrhea, anaemia and can also affects implantation of the foetus.