Effect of Different Levels of Hesperetin on the Quality of Rooster Semen

Abstract

The study examined the impact of varying concentrations of hesperetin on the cryopreservation of rooster sperm. Sperm were diluted with a Lake Extender containing (0, 15, 20, 25, and 30) μM hesperetin. After a 30-second freeze-thaw operation at 37 °C, membrane integrity, sperm motility characteristics, aberrant morphology, apoptotic status, and mitochondrial activity were assessed. The concentration of 25 μM hesperetin observed the highest values ( P < 0.01) were (64.26 %, 30.44 %, 35.88 mm/s, 21.77 mm/s, 61.08 mm/s, 35.66%, 61.14%, 16.23 Hz, 61.71%, 61.71%, and 61.33%) for (Total motility, progressive motility, average velocity path, curved velocity, straight-line velocity, linearity, straightness, beat cross frequency, membrane integrity, mitochondrial activity, and viability, respectively). In contrast, it recorded the lowest values (3.94mm, 13.67%, 13.30%, and 25.37%) for lateral head displacement amplitude, total abnormality, apoptotic sperm, and dead sperm, respectively. It may be concluded that adding 25 μM hesperetin is an effective method for preserving the quality of cryopreserved rooster sperm.