Qualitative and Quantitative Characterization of Secondary Metabolites of Qust' Al-Hindi (Saussurea Lappa) Plants

Nila Tanyela Berghuis, Ana Lailatul Farida, A. Ilmiawati
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Abstract

Utilization of plants that have the potential as herbal medicines, including the Qust' Al-Hindi (Saussurea Lappa) plant. Identification of the secondary metabolite content of the Qust' Al-Hindi Plant (Saussurea Lappa) through Phytochemical analysis in the form of flavonoid, alkaloid, steroid, terpenoid, tannin, and saponin tests on the extraction results as well as the calculation of flavonoid levels which are the largest content of this plant carried out using the UV method. -Vis. The characterization of the presence of a class of secondary metabolites contained in the plant was analyzed using FTIR-ATR spectroscopy. The results of this study indicate that the plant contains secondary metabolites in the form of flavonoid compounds, alkaloids, terpenoids, and tannins. The flavonoid content obtained was 223.33 ± 66.5 mgQE/g, while the results of the FTIR-ATR test showed that the extract contained the same functional groups as flavonoids, alkaloids, terpenoids, and tannins, namely the O-H, C-H, C=C functional groups. C=O and C-O contained in the structure of flavonoids, alkaloid compounds are known to have a distinctive functional group, namely N-H, for terpenoids to have a distinctive functional group, namely C-H, namely CH2 and CH3, there is also CH and there is a C-O group which is a typical functional group of compounds tannins.
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Qust' Al-Hindi(Saussurea Lappa)植物次生代谢物的定性和定量表征
利用具有草药潜力的植物,包括 Qust' Al-Hindi(Saussurea Lappa)植物。通过植物化学分析鉴定 Qust' Al-Hindi 植物(Saussurea Lappa)的次生代谢物含量,包括类黄酮、生物碱、甾体、萜类、单宁和皂苷,对提取结果进行测试,并使用紫外线法计算黄酮含量,黄酮是这种植物中含量最高的物质。-紫外线利用傅立叶变换红外-ATR 光谱分析了该植物所含的一类次生代谢物的特征。研究结果表明,该植物含有黄酮类化合物、生物碱、萜类化合物和单宁酸等形式的次生代谢物。获得的黄酮含量为 223.33 ± 66.5 mgQE/g,而傅立叶变换红外-ATR 测试结果表明,提取物含有与黄酮类化合物、生物碱、萜类化合物和单宁酸相同的官能团,即 O-H、C-H、C=C 官能团。众所周知,黄酮类化合物结构中含有的 C=O 和 C-O,生物碱类化合物有一个独特的官能团,即 N-H,萜类化合物有一个独特的官能团,即 C-H,即 CH2 和 CH3,还有 CH 和一个 C-O 官能团,这是单宁类化合物的典型官能团。
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