Jasmeet Kaur, Sanaa Ismael Abdul Jabar, A. Malik, Humaira Farooqi, Sourab Agarwal, B. Panda
{"title":"In-silico and in-vitro studies of two cannabinoids of Cannabis sativa against prostate cancer","authors":"Jasmeet Kaur, Sanaa Ismael Abdul Jabar, A. Malik, Humaira Farooqi, Sourab Agarwal, B. Panda","doi":"10.31254/jahm.2023.9304","DOIUrl":null,"url":null,"abstract":"The phytochemical profiling of different extracts revealed the presence of high concentration of CBD in 80:20 hydroalcoholic extract, and that of THC in 60:40 hydroalcoholic extract. The MTT assay of combination of CBD and THC highlighted the extracts as potent cytotoxic agents against prostate cancer (PC3) cells, with IC50 values of 1292 ng/ml, 953.3 ng/ml and 1134 ng/ml, respectively for HCP-CO4, HCM-CO1 & HCZ-CO1. The molecular docking study revealed a good binding of androgen receptor (PDB ID, 2am9) with CBD and THC possessing binding affinity energy of -7.1 and -7.2 kcal/mol respectively. Based on the amino acid residual interaction of CBD and THC within the 2am9 receptor, THC reported additional hydrogen bonds as compared to CBD, suggesting it to be more potent antagonist in comparison to CBD. The present study highlighted the potential of CBD and THC as a therapeutic agent for treatment of prostate cancer.","PeriodicalId":15252,"journal":{"name":"Journal of Ayurvedic and Herbal Medicine","volume":"119 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Ayurvedic and Herbal Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.31254/jahm.2023.9304","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
The phytochemical profiling of different extracts revealed the presence of high concentration of CBD in 80:20 hydroalcoholic extract, and that of THC in 60:40 hydroalcoholic extract. The MTT assay of combination of CBD and THC highlighted the extracts as potent cytotoxic agents against prostate cancer (PC3) cells, with IC50 values of 1292 ng/ml, 953.3 ng/ml and 1134 ng/ml, respectively for HCP-CO4, HCM-CO1 & HCZ-CO1. The molecular docking study revealed a good binding of androgen receptor (PDB ID, 2am9) with CBD and THC possessing binding affinity energy of -7.1 and -7.2 kcal/mol respectively. Based on the amino acid residual interaction of CBD and THC within the 2am9 receptor, THC reported additional hydrogen bonds as compared to CBD, suggesting it to be more potent antagonist in comparison to CBD. The present study highlighted the potential of CBD and THC as a therapeutic agent for treatment of prostate cancer.
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