{"title":"Screening of Inhibitory Effects Lanosterol on Butyrylcholinesterase, Acetylcholinesterase and Carbonic Anhydrase Enzymes","authors":"E. Üç","doi":"10.21597/jist.1308662","DOIUrl":null,"url":null,"abstract":"In this study, for the first time, the antiglaucoma and anticholinergic properties from lanosterol were appraised and researched using different bioanalytical methods and compared with standards. Lanosterol is the compound from which entire fungal and animal steroids are derived. Moreover, is a tetracyclic triterpenoid. Lanosterol is a component in over-the-counter ophthalmic products to prohibit cataracts. The inhibition effects of lanosterol were tested against the butyrylcholinesterase (BChE), carbonic anhydrase I and II (CA I and II) and acetylcholinesterase (AChE), which are associated with some global diseases like Alzheimer’s disease (AD) and glaucoma. Lanosterol were trialed for the inhibition of BChE, AChE, hCA I and II enzymes and indicated efficient inhibition profiles with Ki values in the range of 61.77±22.32 nM against hCA I, 101.11±49.74 nM against hCA II, 2.03±1.21 nM against acetylcholinesterase and 8.39±2.92 nM against butyrylcholinesterase.","PeriodicalId":17353,"journal":{"name":"Journal of the Institute of Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of the Institute of Science and Technology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21597/jist.1308662","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, for the first time, the antiglaucoma and anticholinergic properties from lanosterol were appraised and researched using different bioanalytical methods and compared with standards. Lanosterol is the compound from which entire fungal and animal steroids are derived. Moreover, is a tetracyclic triterpenoid. Lanosterol is a component in over-the-counter ophthalmic products to prohibit cataracts. The inhibition effects of lanosterol were tested against the butyrylcholinesterase (BChE), carbonic anhydrase I and II (CA I and II) and acetylcholinesterase (AChE), which are associated with some global diseases like Alzheimer’s disease (AD) and glaucoma. Lanosterol were trialed for the inhibition of BChE, AChE, hCA I and II enzymes and indicated efficient inhibition profiles with Ki values in the range of 61.77±22.32 nM against hCA I, 101.11±49.74 nM against hCA II, 2.03±1.21 nM against acetylcholinesterase and 8.39±2.92 nM against butyrylcholinesterase.
本研究首次使用不同的生物分析方法对羊毛甾醇的抗青光眼和抗胆碱能特性进行了评估和研究，并与标准进行了比较。羊毛甾醇是所有真菌和动物甾体化合物的来源。此外，它还是一种四环三萜类化合物。羊毛甾醇是非处方药眼科产品中的一种成分，用于预防白内障。测试了羊毛甾醇对丁酰胆碱酯酶（BChE）、碳酸酐酶 I 和 II（CA I 和 II）以及乙酰胆碱酯酶（AChE）的抑制作用。对羊毛甾醇进行了抑制 BChE、AChE、hCA I 和 II 酶的试验，结果表明其具有高效的抑制作用，对 hCA I 的 Ki 值为 61.77±22.32 nM，对 hCA II 的 Ki 值为 101.11±49.74 nM，对乙酰胆碱酯酶的 Ki 值为 2.03±1.21 nM，对丁酰胆碱酯酶的 Ki 值为 8.39±2.92 nM。
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