Sex modulates the diet-induced changes to the plasma lipidome in a rat model of cardiorespiratory fitness

IF 3.9 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-01-06 DOI:10.1016/j.bbalip.2024.159451
Johanna Y. Fleischman , James L. Casey , Jennifer L. Meijer , Mary K. Treutelaar , Thekkelnaycke M. Rajendiran , Tanu Soni , Charles R. Evans , Charles F. Burant
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Abstract

Objective

Individuals with higher intrinsic cardiorespiratory fitness (CRF) experience decreased rates of cardiometabolic disease and mortality, and high CRF is associated with increased utilization of fatty acids (FAs) for energy. Studies suggest a complex relationship between CRF, diet, and sex with health outcomes, but this interaction is understudied. We hypothesized that FA utilization differences by fitness and sex could be detected in the plasma metabolome when rats or humans were fed a high carbohydrate (HC) or high fat (HF) diet.

Methods

Male and female rats selectively bred for low (LCR) and high (HCR) CRF were fed a chow diet or a sucrose-free HF (45 % fat) or HC (10 % fat) diet. Plasma samples were collected at days 0, 3, and 14. Human plasma data was collected from male and female participants who were randomized into a HC or HF diet for 21 days. Samples were analyzed using liquid chromatography-mass spectrometry and regression statistics were used to quantify the effect of diet, CRF, and sex on the lipidome.

Results

In rats, the baseline lipidome is more significantly influenced by sex than by CRF, especially as elevated diglycerides, triglycerides, phosphatidylcholines, and lysophosphatidylcholines in males. A dynamic response to diet was observed 3 days after diet, but after 14 days of either diet, the lipidome was modulated by sex with a larger effect size than by diet. Data from the human study also suggests a sex-dependent response to diet with opposite directionality of affect compared to rats, highlighting species-dependent responses to dietary intervention.

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在大鼠心肺功能模型中,性别可调节饮食引起的血浆脂质体变化
目的具有较高内在心肺功能(CRF)的人患心脏代谢疾病的比率和死亡率都会降低,而高CRF与脂肪酸(FA)能量利用率的提高有关。研究表明,CRF、饮食和性别与健康结果之间存在复杂的关系,但对这种相互作用的研究还不够。我们假设,在给大鼠或人类喂食高碳水化合物(HC)或高脂肪(HF)饮食时,可以在血浆代谢组中检测到不同体质和性别的脂肪酸利用率差异。方法选择性繁殖低(LCR)和高(HCR)CRF的雄性和雌性大鼠,喂食饲料或不含蔗糖的HF(45%脂肪)或HC(10%脂肪)饮食。在第 0、3 和 14 天收集血浆样本。从随机接受 HC 或 HF 饮食 21 天的男性和女性参与者中收集人体血浆数据。结果在大鼠中,基线脂质组受性别的影响比受 CRF 的影响更显著,尤其是雄性大鼠的二甘油酯、甘油三酯、磷脂酰胆碱和溶血磷脂酰胆碱升高。饮食 3 天后可观察到对饮食的动态反应,但任何一种饮食 14 天后,脂质组受性别调节的效应比受饮食调节的效应大。人类研究的数据还表明,与大鼠相比,人类对饮食的反应与性别有关,其影响方向相反,这突出表明饮食干预的反应与物种有关。
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来源期刊
CiteScore
11.00
自引率
2.10%
发文量
109
审稿时长
53 days
期刊介绍: BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.
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