Relationship between the cytokine profle of supernatants of invasive breast carcinoma and its molecular and histopathological characteristics

A. Autenshlyus, S. Arkhipov, E. Mikhaylova, V. Arkhipova, N. Varaksin
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Abstract

The aim of the study was to analyze the correlation between the cytokine profile of supernatants of invasive breast carcinoma of a nonspecific type (IBC-NST) samples, histopathological and molecular genetic parameters of IBC-NST, expression of the CD34 as a marker of angiogenesis and metastasis to regional lymph nodes (RLN).Material and Methods. The production of 14 cytokines in IBC-NST biopsy samples from 28 patients aged 37–60 years was studied. The concentration of cytokines in the supernatants of biopsies (CCSB) was determined (in pg/ml) using enzyme immunoassay (ELISA). The expression of CD34 and markers of IBC-NST molecular subtypes (HER2/neu, ER, PR, Ki67) in IBC-NST biopsy samples was evaluated by immunohistochemical method. The relative content of tumor cells of different differentiation grade in the IBC- NST samples was evaluated by histopathological analysis.Results. The assessment of CCSB showed statistically significant differences in IFN-γ, G-CSF, IL-2, IL-10 and MCP-1 between patients of group I (with metastases in RLNs) and group II (without metastases in RLNs). In group I, the correlations between histopathological parameters (Her2/neu, CD34 and Ki67 expressions, % of mitoses and poorly-differentiated cancer cells) and CCSB (MCP-1, IL-18) were revealed. In group II, the correlations between CCSB (IL-2, VEGF-A, G-CSF, IL-1Ra) and histopathological parameters, such as expression of Her2/neu, CD34, PR, % of mitoses and well-differentiated cancer cells, were revealed. The ROC analysis showed that the presence or absence of metastases in RLNs can be predicted on the basis of CD34 expression levels and concentrations of IL-10, G-CSF, and MCP-1 in supernatants of IBC-NST biopsy samples. The quality of the model for stratifying patients into groups with and without RLN metastases, based on the assessment of the concentration of MCP-1 in the supernatants of IBC-NST biopsies, reached maximum values (AUC=1.000) with relatively high CD34 expression.Conclusion. The analysis of the data obtained showed that the assessment of CD34 expression and production of cytokines in IBC-NST biopsies is important for predicting the presence or absence of metastases in RLNs.
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浸润性乳腺癌上清液中的细胞因子概况与其分子和组织病理学特征之间的关系
该研究旨在分析非特异性浸润性乳腺癌(IBC-NST)样本上清液中细胞因子谱、IBC-NST的组织病理学和分子遗传学参数、作为血管生成和区域淋巴结(RLN)转移标志物的CD34的表达之间的相关性。研究了 28 名 37-60 岁 IBC-NST 患者活检样本中 14 种细胞因子的产生情况。使用酶联免疫吸附法(ELISA)测定活检上清液(CCSB)中细胞因子的浓度(以 pg/ml 为单位)。免疫组化法评估了 IBC-NST 活检样本中 CD34 和 IBC-NST 分子亚型标记物(HER2/neu、ER、PR、Ki67)的表达。组织病理学分析评估了 IBC-NST 样本中不同分化等级肿瘤细胞的相对含量。CCSB评估结果显示,I组(RLN有转移)和II组(RLN无转移)患者的IFN-γ、G-CSF、IL-2、IL-10和MCP-1差异有统计学意义。在 I 组中,组织病理学参数(Her2/neu、CD34 和 Ki67 表达、有丝分裂和低分化癌细胞百分比)与 CCSB(MCP-1、IL-18)之间存在相关性。在第二组中,CCSB(IL-2、VEGF-A、G-CSF、IL-1Ra)与组织病理学参数(如 Her2/neu、CD34、PR、有丝分裂百分比和分化良好的癌细胞的表达)之间的相关性显现出来。ROC分析表明,根据CD34的表达水平以及IBC-NST活检样本上清液中IL-10、G-CSF和MCP-1的浓度,可以预测RLN中是否存在转移。根据对 IBC-NST 活检样本上清液中 MCP-1 浓度的评估,将患者分为有和无 RLN 转移组的模型质量在 CD34 表达相对较高时达到了最大值(AUC=1.000)。对所获数据的分析表明,评估 IBC-NST 活检组织中 CD34 的表达和细胞因子的产生对于预测 RLN 是否存在转移非常重要。
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来源期刊
Siberian journal of oncology
Siberian journal of oncology Medicine-Oncology
CiteScore
0.40
自引率
0.00%
发文量
117
审稿时长
8 weeks
期刊介绍: The main objectives of the journal are: -to promote the establishment of Russia’s leading worldwide positions in the field of experimental and clinical oncology- to create the international discussion platform intended to cover all aspects of basic and clinical cancer research, including carcinogenesis, molecular biology, epidemiology, cancer prevention, diagnosis and multimodality treatment (surgery, chemotherapy, radiation therapy, hormone therapy), anesthetic management, medical and social rehabilitation, palliative care as well as the improvement of life quality of cancer patients- to encourage promising young scientists to be actively involved in cancer research programs- to provide a platform for researches and doctors all over the world to promote, share, and discuss various new issues and developments in cancer related problems. (to create a communication platform for the expansion of cooperation between Russian and foreign professional associations).- to provide the information about the latest worldwide achievements in different fields of oncology The most important tasks of the journal are: -to encourage scientists to publish their research results- to offer a forum for active discussion on topics of major interest - to invite the most prominent Russian and foreign authors to share their latest research findings with cancer research community- to promote the exchange of research information, clinical experience, current trends and the recent developments in the field of oncology as well as to review interesting cases encountered by colleagues all over the world- to expand the editorial board and reviewers with the involvement of well-known Russian and foreign experts- to provide open access to full text articles- to include the journal into the international database- to increase the journal’s impact factor- to promote the journal to the International and Russian markets
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