Expression of allergenic protein and polycyclic aromatic hydrocarbons in the Platanus pollen grains collected from different area of Shanghai

IF 2.2 3区环境科学与生态学 Q2 BIOLOGY Aerobiologia Pub Date : 2024-01-16 DOI:10.1007/s10453-023-09805-7

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Abstract

As one of biological particles, pollen grains could be suspended in air and cause health risks such as pollinosis and rhinitis. Air pollutants can be absorbed on the pollen grains which would increase allergenicity. However, few studies have been focused on characterization of polycyclic aromatic hydrocarbons (PAHs), one kind of toxic pollutants absorbed on pollen grains, and their relationships with the allergenic proteins. In this study, fresh Platanus pollen samples were collected in the central parking area and the outer ring area of Shanghai in the spring of 2023. Mass concentration of allergenic protein (Pla a3) released from the samples collected at the two sampling sites were investigated by using western blot assay and enzyme linked immunosorbent assay, while mass levels of PAHs absorbed on the surface of flower inflorescence and pollen grains were measured by using GC-MS. Our data showed that the mass level of Pla a3 released from the pollen grains collected in the parking area was significantly higher than that collected in the outer ring area. Mass level of PAHs on the flower inflorescence and pollen grains in the parking area was 2 times higher than that in the outer ring area. The molecular docking results indicated that four disulfide bonds (Cys4-Cys52, Cys14-Cys29, Cys30-Cys75, Cys50-Cys89) in the Pla a3 protein structure could interact with the PAHs by hydrogen bonds. This interaction could increase the structural stability of the protein and enhance the allergenicity.

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上海不同地区采集的桔梗花粉颗粒中过敏原蛋白和多环芳烃的表达

摘要花粉粒作为生物微粒之一，可悬浮在空气中，对人体健康造成危害，如花粉症和鼻炎。空气污染物会被花粉粒吸收，从而增加过敏性。多环芳烃（PAHs）是花粉粒上吸收的一种有毒污染物，但很少有研究关注多环芳烃的特性及其与致敏蛋白的关系。本研究于 2023 年春季在上海中心停车区和外环区域采集了新鲜的桔梗花粉样本。采用 Western 印迹分析法和酶联免疫吸附分析法对两个采样点采集的样品中释放的过敏原蛋白（Pla a3）的质量浓度进行了研究，同时采用气相色谱-质谱法测定了花序和花粉粒表面吸收的多环芳烃的质量水平。数据显示，从停车区采集的花粉粒中释放出的 Pla a3 的质量水平明显高于从外环区采集的花粉粒中释放出的 Pla a3 的质量水平。停车区花序和花粉粒上 PAHs 的质量水平是外环区的 2 倍。分子对接结果表明，Pla a3 蛋白结构中的四个二硫键（Cys4-Cys52、Cys14-Cys29、Cys30-Cys75、Cys50-Cys89）可通过氢键与多环芳烃相互作用。这种相互作用可增加蛋白质的结构稳定性，并增强其致敏性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aerobiologia 环境科学-环境科学

CiteScore

4.50

自引率

15.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Associated with the International Association for Aerobiology, Aerobiologia is an international medium for original research and review articles in the interdisciplinary fields of aerobiology and interaction of human, plant and animal systems on the biosphere. Coverage includes bioaerosols, transport mechanisms, biometeorology, climatology, air-sea interaction, land-surface/atmosphere interaction, biological pollution, biological input to global change, microbiology, aeromycology, aeropalynology, arthropod dispersal and environmental policy. Emphasis is placed on respiratory allergology, plant pathology, pest management, biological weathering and biodeterioration, indoor air quality, air-conditioning technology, industrial aerobiology and more. Aerobiologia serves aerobiologists, and other professionals in medicine, public health, industrial and environmental hygiene, biological sciences, agriculture, atmospheric physics, botany, environmental science and cultural heritage.