microRNAs associated with the quality of follicular fluids affect oocyte and early embryonic development.

IF 3.3 3区医学 Q2 OBSTETRICS & GYNECOLOGY Reproductive Medicine and Biology Pub Date : 2024-01-18 eCollection Date: 2024-01-01 DOI:10.1002/rmb2.12559

Sogo Aoki, Yuki Inoue, Shunsuke Hara, Jun Itou, Koumei Shirasuna, Hisataka Iwata

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Abstract

Purpose: Oocyte and embryo quality differs significantly among individuals. Follicular fluid (FF) is a solo environment of oocyte maturation and may flux into the oviduct. Supplementation of in vitro maturation (IVM) and culture (IVC) medium with extracellular vesicles of FFs supports oocyte maturation and embryonic development. We addressed a hypothesis that miRNA profiles in FFs are crucial background of oocyte maturation and embryonic development.

Methods: FFs were collected from the ovaries of individual cows, and the FFs were classified into Good or Poor FF based on the developmental rate to the blastocyst stage of enclosed oocytes. miRNAs associated with the Good FFs were explored using small RNA sequencing. In addition, FFs were classified using the concentration of Good-FF-associated miRNAs. These classified FFs or miRNA were added to the IVM or IVC mediums.

Results: Supplementation of IVM and IVC medium with Good FF improved embryonic development. Good FFs contained miR-151-3p and miR-425-5p at a high concentration compared with those in Poor FFs. FFs selected by the concentration of miR-151-3p and miR-425-5p improved oocyte maturation and embryonic development. Supplementation of IVM or IVC medium with either miR-151-3p or miR-425-5p improved embryonic development to the blastocyst stage.

Conclusion: miRNAs were associated with the Good FFs determined oocyte maturation and embryonic development.

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与卵泡液质量有关的 microRNA 会影响卵母细胞和早期胚胎发育。

目的：不同个体的卵母细胞和胚胎质量差异很大。卵泡液（FF）是卵母细胞成熟的唯一环境，并可能流入输卵管。在体外成熟（IVM）和培养（IVC）培养基中添加卵泡液的胞外囊泡有助于卵母细胞成熟和胚胎发育。我们提出了一个假设：FFs 中的 miRNA 图谱是卵母细胞成熟和胚胎发育的重要背景：方法：从个体奶牛的卵巢中收集 FFs，并根据被包被卵母细胞发育到囊胚期的速度将 FFs 分为好 FFs 和差 FFs。此外，还利用与好 FF 相关的 miRNA 的浓度对 FF 进行了分类。将这些分类的 FF 或 miRNA 添加到 IVM 或 IVC 培养基中：结果：在IVM和IVC培养基中添加Good FF能改善胚胎发育。与差FFs相比，好FFs含有高浓度的miR-151-3p和miR-425-5p。根据 miR-151-3p 和 miR-425-5p 的浓度选择的 FFs 可改善卵母细胞成熟和胚胎发育。结论：miRNA 与决定卵母细胞成熟和胚胎发育的良好 FFs 有关。

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来源期刊

Reproductive Medicine and Biology Multiple-

CiteScore

5.70

自引率

5.90%

发文量

审稿时长

20 weeks

期刊介绍： Reproductive Medicine and Biology (RMB) is the official English journal of the Japan Society for Reproductive Medicine, the Japan Society of Fertilization and Implantation, the Japan Society of Andrology, and publishes original research articles that report new findings or concepts in all aspects of reproductive phenomena in all kinds of mammals. Papers in any of the following fields will be considered: andrology, endocrinology, oncology, immunology, genetics, function of gonads and genital tracts, erectile dysfunction, gametogenesis, function of accessory sex organs, fertilization, embryogenesis, embryo manipulation, pregnancy, implantation, ontogenesis, infectious disease, contraception, etc.