Biogeochemical and microbial community structure differently modulates CO2 and CH4 dynamics in two adjacent volcanic lakes (Monticchio, Italy)

Abstract

By hosting significant amounts of extra-atmospheric dissolved gases, including geogenic CO₂ and CH₄, volcanic lakes provide relevant ecosystem services through the key role the aquatic microbial community in mediating freshwater carbon fluxes. In view of elucidating the mechanisms governing the microbial spatial distribution and the possible implications for ecosystem functioning, we compared the hydrogeochemical features and the microbial community structure of two adjacent stratified volcanic lakes (Lake Grande - LG and Lake Piccolo - LP). Water chemistry, gases and their isotopic composition were coupled with microbial pigment profiling, cell counting, and phylogenetic analyses. LP showed transparent waters with low concentrations of chlorophyll-a and the occurrence of phycoerytrin-rich cyanobacteria. LG was relatively more eutrophic with a higher occurrence of diatoms and phycocyanine-rich cyanobacteria. Considering the higher concentrations of CO₂ and CH₄ in bottom waters, the oligotrophic LP was likely a more efficient sink of geogenic CO₂ in comparison to the adjacent eutrophic LG. The prokaryotic community was dominated by the mixothrophic hgcI clade (family Sporichthyaceae) in the LG surface waters, while in LP this taxon was dominant down to -15 m. Moreover, in LP, the bottom dark waters harbored a unique strictly anaerobic bacterial assemblage associated with methanogenic Archaea (i.e. Methanomicrobiales), resulting in a high biogenic methane concentration. Water layering and light penetration were confirmed as major factors affecting the microbial distribution patterns. The observed differences in the geochemical and trophic conditions reflected the structure of the aquatic microbial community, with direct consequences on the dynamics of dissolved greenhouse gases.