Using RNA interference targeting a nicotinic acetylcholine receptor subunit to counteract insecticide accommodation mechanisms: example of the β1 subunit in the imidacloprid-accommodated American cockroach, Periplaneta americana

IF 1.5 4区 农林科学 Q2 ENTOMOLOGY Journal of Pesticide Science Pub Date : 2024-01-30 DOI:10.1584/jpestics.d23-027
Sebastien Ligonniere, Alexandre Bantz, Valerie Raymond, Delphine Goven
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Abstract

Insecticide accommodation and resistance are limiting factors to the much-needed increase in agricultural production. Various physiological and cellular modifications, such as the changes of insecticide molecular targets, have been linked to these events. Thus, a previous study demonstrated that the imidacloprid accommodation set up by the cockroach Periplaneta americana after an exposure to a sublethal dose of this insecticide involves functional alterations of two nicotinic acetylcholine receptor (nAChR) subtypes. As RNA interference (RNAi) is one of the most promising strategies for controlling pest insects, we evaluated, in this study, the use of RNAi that targets the β1 nAChR subunit to counteract the imidacloprid accommodation phenomenon in cockroaches. Interestingly, we showed that ingestion of dsRNA-β1 increased the sensitivity to imidacloprid of accommodated cockroaches. Thus, we have demonstrated for the first time that RNAi that targets an nAChR subunit can counteract the accommodation mechanism to insecticide targeting nAChRs set up by an insect.

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利用针对烟碱乙酰胆碱受体亚基的 RNA 干扰来对抗杀虫剂适应机制:以吡虫啉适应美洲大蠊的 β1 亚基为例
杀虫剂的适应性和抗药性是亟需提高的农业产量的限制因素。各种生理和细胞变化,如杀虫剂分子靶标的变化,都与这些事件有关。因此,先前的一项研究表明,蟑螂 Peridiplaneta americana 在接触亚致死剂量的吡虫啉杀虫剂后建立的适应性涉及两种烟碱乙酰胆碱受体(nAChR)亚型的功能改变。由于 RNA 干扰(RNAi)是控制有害昆虫的最有前途的策略之一,我们在本研究中评估了使用靶向 β1 nAChR 亚基的 RNAi 来对抗蟑螂的吡虫啉宿主现象。有趣的是,我们发现摄入 dsRNA-β1 会增加迁入蟑螂对吡虫啉的敏感性。因此,我们首次证明了靶向 nAChR 亚基的 RNAi 能够抵消昆虫建立的对杀虫剂靶向 nAChR 的适应机制。
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来源期刊
Journal of Pesticide Science
Journal of Pesticide Science 农林科学-昆虫学
CiteScore
4.30
自引率
4.20%
发文量
28
审稿时长
18-36 weeks
期刊介绍: The Journal of Pesticide Science publishes the results of original research regarding the chemistry and biochemistry of pesticides including bio-based materials. It also covers their metabolism, toxicology, environmental fate and formulation.
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