Determination of nobiletin and tangeretin in rat plasma by ultra-performance liquid chromatography-tandem mass spectrometry and study of their pharmacokinetics

IF 17.7 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-01-23 DOI:10.1556/1326.2024.01158

Aiguo Zhang, Ziyue Wang, Wenqian Ding, Dizhong Chen, Shunjun Ma, Congcong Wen, Xianqin Wang, Yongxi Jin, Yinghao Zhi

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Abstract

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of nobiletin and tangeretin in rat plasma, and the plasma was processed by a simple liquid-liquid extraction method with ethyl acetate. The chromatographic column was UPLC HSS T3 (50 × 2.1 mm, 1.7 μm), the mobile phase was acetonitrile-water (containing 0.1% formic acid). Multiple reaction monitoring mode (MRM) was used for quantitative analysis, nobiletin m/z 403.29 → 373.14 (cone voltage 22v, collision voltage 28v), tangeretin m/z 373.28 → 343.17 (cone voltage 20v, collision voltage 28V), tangeretin m/z 373.28 → 343.17 (cone voltage 20V, collision voltage 28V) and internal standard vitexin m/z 433.14 → 313.03 (cone voltage 32v, collision voltage 26v). The pharmacokinetics of nobiletin and tangeretin were evaluated in rats. The established UPLC-MS/MS method in the range of 2–2,000 ng mL−1 was successfully applied to the pharmacokinetics, and the calculated bioavailability of nobiletin and tangeretin was 63.9 and 46.1%, respectively.

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超高效液相色谱-串联质谱法测定大鼠血浆中的金雀花素和桔皮素并研究其药代动力学

建立了大鼠血浆中金钗素和桔梗素的超高效液相色谱-串联质谱检测方法。色谱柱为UPLC HSS T3（50×2.1 mm，1.7 μm），流动相为乙腈-水（含0.1%甲酸）。采用多反应监测模式（MRM）进行定量分析，nobiletin m/z 403.29 → 373.14（锥电压 22v，碰撞电压 28v），tangeretin m/z 373.28 → 343.17（锥体电压 20V，碰撞电压 28V），桔皮素 m/z 373.28 → 343.17（锥体电压 20V，碰撞电压 28V）和内标桔皮素 m/z 433.14 → 313.03（锥体电压 32V，碰撞电压 26V）。在大鼠体内对 nobiletin 和 tangeretin 的药代动力学进行了评估。所建立的 UPLC-MS/MS 方法在 2-2,000 ng mL-1 的范围内成功地应用于药代动力学研究，计算出的新生物利用率分别为 63.9%和 46.1%。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

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