Antimicrobial activity of hydralazine against methicillin-resistant and methicillin-susceptible Staphylococcus aureus.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS ACS Applied Bio Materials Pub Date : 2024-01-01 Epub Date: 2024-01-31 DOI:10.2217/fmb-2023-0160

Francisca B Stefany Aires do Nascimento, Lívia Gurgel do Amaral Valente Sá, João B de Andrade Neto, Lisandra Juvêncio da Silva, Daniel Sampaio Rodrigues, Vitória P de Farias Cabral, Amanda Dias Barbosa, Lara E Almeida Moreira, Camille R Braga Vasconcelos, Bruno Coêlho Cavalcanti, Maria E França Rios, Jacilene Silva, Emmanuel Silva Marinho, Helcio Silva Dos Santos, Jacó Rl de Mesquita, Marina Duarte Pinto Lobo, Manoel Odorico de Moraes, Hélio V Nobre Júnior, Cecília Rocha da Silva

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Abstract

Background: Staphylococcus aureus is a human pathogen responsible for high mortality rates. The development of new antimicrobials is urgent. Materials & methods: The authors evaluated the activity of hydralazine along with its synergism with other drugs and action on biofilms. With regard to action mechanisms, the authors evaluated cell viability, DNA damage and molecular docking. Results: MIC and minimum bactericidal concentration values ranged from 128 to 2048 μg/ml. There was synergism with oxacillin (50%) and vancomycin (25%). Hydralazine reduced the viability of biofilms by 50%. After exposure to hydralazine 2× MIC, 58.78% of the cells were unviable, 62.07% were TUNEL positive and 27.03% presented damage in the comet assay (p < 0.05). Hydralazine showed affinity for DNA gyrase and TyrRS. Conclusion: Hydralazine is a potential antibacterial.

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肼屈嗪对耐甲氧西林和甲氧西林敏感金黄色葡萄球菌的抗菌活性。

背景：金黄色葡萄球菌是导致高死亡率的人类病原体。开发新的抗菌药物迫在眉睫。材料与方法：作者评估了肼屈嗪的活性及其与其他药物的协同作用和对生物膜的作用。关于作用机制，作者评估了细胞活力、DNA 损伤和分子对接。结果如下MIC 和最低杀菌浓度值介于 128 至 2048 μg/ml 之间。与奥沙西林（50%）和万古霉素（25%）有协同作用。肼屈嗪可将生物膜的存活率降低 50%。暴露于 2 倍 MIC 的肼屈嗪后，58.78% 的细胞无法存活，62.07% 的细胞呈 TUNEL 阳性，27.03% 的细胞在彗星试验中出现损伤（p 结论：肼屈嗪是一种潜在的抗菌剂：肼屈嗪是一种潜在的抗菌剂。

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