Biological and physical properties of calcium hydroxide-based pulp-capping materials and their modifications.

IF 1.9 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE Journal of oral science Pub Date : 2024-04-16 Epub Date: 2024-02-08 DOI:10.2334/josnusd.23-0288

Dilek Akin, Cigdem Atalayin Ozkaya, Guliz Armagan, Dervis Birim, Mustafa Ates, Huseyin Tezel

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Abstract

Purpose: To evaluate the biological and physical properties of calcium hydroxide-containing pulp-capping materials and their modifications with different solutions and antioxidant Resveratrol (RES) addition.

Methods: Calcium hydroxide+distilled-water:C, calcium hydroxide+saline:S, calcium hydroxide+synthetic tissue fluid:STF, Dycal:D, calcium hydroxide+distilled-water+RES:C+RES, calcium hydroxide+saline+RES:S+RES, calcium hydroxide+synthetic tissue fluid+RES:STF+RES, Dycal+RES:D+RES were tested. Cytotoxicity was determined by WST-1. Antibacterial-activity was evaluated by agar-diffusion. The water-absorption and solubility were examined for ISO-6876 and ISO-3107. The color-change was evaluated by spectrophotometer. Radiopacity was evaluated for ISO-6876 and ISO-9917. The normal distribution and homogeneity were determined and comparisons were made with appropriate analysis and post hoc tests (P < 0.05).

Results: The highest cell-viability was determined in the C+RES and the lowest was in D and D+RES after 24 h (P < 0.0001). RES-addition increased cell-viability and the highest rate was detected in C+RES, S+RES and STF+RES after 48 h (P < 0.0001). A limited inhibition-zone against Streptococcus mutans was detected in D and D+RES. RES-addition did not change the water-absorption in S and STF or the solubility in S group.

Conclusion: RES-addition may be used to increase the biocompatibility of calcium hydroxide without any adverse effect on physical properties. Saline may be the first choice as a mixing solution.

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基于氢氧化钙的纸浆盖材料及其改性的生物和物理特性。

目的：评估含氢氧化钙的牙髓覆盖材料的生物和物理特性，以及不同溶液和抗氧化剂白藜芦醇（RES）对其的改性：氢氧化钙+蒸馏水:C、氢氧化钙+生理盐水:S、氢氧化钙+合成组织液:STF、Dycal:D、氢氧化钙+蒸馏水+RES:C+RES、氢氧化钙+生理盐水+RES:S+RES、氢氧化钙+合成组织液+RES:STF+RES、Dycal+RES:D+RES。细胞毒性由 WST-1 测定。抗菌活性通过琼脂扩散法进行评估。ISO-6876 和 ISO-3107 检测了吸水性和溶解性。用分光光度计评估颜色变化。对 ISO-6876 和 ISO-9917 进行了不透射线性评估。测定了正态分布和均匀性，并通过适当的分析和事后检验（P < 0.05）进行了比较：结果：24 小时后，C+RES 的细胞存活率最高，D 和 D+RES 的细胞存活率最低（P < 0.0001）。添加 RES 可提高细胞活力，48 小时后，C+RES、S+RES 和 STF+RES 的细胞活力最高（P < 0.0001）。在 D 和 D+RES 中检测到了对变异链球菌的有限抑制区。添加 RES 不会改变 S 组和 STF 组的吸水性或 S 组的溶解性：结论：添加 RES 可用于提高氢氧化钙的生物相容性，而不会对物理性质产生任何不利影响。生理盐水可作为混合溶液的首选。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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