Nematicidal activity of paucimannose-type glycoconjugates from acacia honey

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-02-07 DOI:10.1016/j.exppara.2024.108707

Bushra Bilal , M. Kamran Azim

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Abstract

Natural honey contains glycoconjugates as minor components. We characterized acacia honey glycoconjugates with molecular masses in the range of 2–5 kDa. The glycoconjugates were separated by RP-HPLC into three peaks (termed RP-2-5 k-I, RP-2-5 k-II, and RP-2-5 k-III) which demonstrated paralyzing effects on the model nematode C. elegans (ED₅₀ of 50 ng glycoconjugates/μL). To examine molecular mechanisms underlying the nematicidal effects of honey glycoconjugates, expressional analyses of genes that are essential for the growth, development, reproduction, and movement of C. elegans were carried out. Quantitative PCR-based assays showed that these molecules moderately regulate the expression of genes involved in the citric acid cycle (mdh-1 and idhg-1) and cytoskeleton (act-1 and act-2). MALDI-ToF-MS/MS analysis of RP-HPLC peaks revealed the presence of paucimannose-like N-glycans which are known to play important roles in invertebrates e.g., worms and flies. These findings provided novel information regarding the structure and nematicidal function of honey glycoconjugates.

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刺槐蜜中的白芒果糖型糖苷键合物的杀线虫活性。

天然蜂蜜中含有作为次要成分的糖结合物。我们对分子质量在 2-5 kDa 范围内的洋槐蜜糖结合物进行了鉴定。通过 RP-HPLC 将糖结合物分离成三个峰（分别称为 RP-2-5 k-I、RP-2-5 k-II 和 RP-2-5 k-III），这些糖结合物对模式线虫秀丽隐杆线虫具有麻痹作用（ED50 为 50 ng 糖结合物/μL）。为了研究蜂蜜糖苷酸杀线虫作用的分子机制，我们对线虫的生长、发育、繁殖和运动所必需的基因进行了表达分析。基于定量 PCR 的分析表明，这些分子可适度调节柠檬酸循环（mdh-1 和 idhg-1）和细胞骨架（act-1 和 act-2）相关基因的表达。对 RP-HPLC 峰进行的 MALDI-ToF-MS/MS 分析表明，存在类似于 paucimannose 的 N-聚糖，众所周知，这些 N-聚糖在无脊椎动物（如蠕虫和苍蝇）中发挥着重要作用。这些发现提供了有关蜂蜜糖共轭物的结构和杀线虫功能的新信息。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.