Recent sequencing and phylogenetic analysis of equine herpesviruses 1 and 4 among different equine populations in Egypt.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2023-12-31 eCollection Date: 2023-12-01 DOI:10.5455/javar.2023.j719

Mohamed El-Zayat, Omayma A Shemies, Samah M Mosad, Sahar Abd El Rahman

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Abstract

Objective: Equine herpes viruses (EHVs) are considered one of the most important respiratory pathogens in equids, resulting in serious outcomes for equine health worldwide. The objectives of the current research were the detection, molecular characterization, and isolation of EHV-1 and EHV-4 circulating within different equine populations in Egypt, either clinically or in apparently healthy horses.

Material and methods: A total of 120 field samples were collected, and DNA was extracted. Screening and typing of extracted DNA were done by consensus and conventional PCR assays for detection of EHV-1 and EHV-4, followed by sequencing and phylogenetic analysis to confirm the virus identity. Selected positive samples for both EHV-1 and EHV-4 were subjected to Madin-Darby bovine kidney (MDBK) cell lines for virus isolation.

Results: The obtained results revealed that 58/120 (48%) samples were positive for EHVs. Typing of positive samples showed that EHV-1 was detected in (48/120) 40% of samples and EHV-4 was detected in (15/120) 12% of samples, while dual infection by both EHV-1 and 4 was detected in five samples.

Conclusion: The current study revealed new data on the continuous circulation of EHV-1 and EHV-4 within equine populations in Egypt, and individual horses could be infected by multiple EHVs. In addition, latently infected horses are acting as potential reservoirs for frequent virus reactivation.

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埃及不同马群中马疱疹病毒 1 和 4 的最新测序和系统发育分析。

目的：马疱疹病毒（EHV）被认为是马类最重要的呼吸道病原体之一，对全世界的马健康造成了严重后果。本次研究的目的是检测、分子鉴定和分离埃及不同马群中流行的 EHV-1 和 EHV-4，无论是临床上还是表面上健康的马：材料和方法：共收集了 120 份现场样本，并提取了 DNA。通过共识和传统 PCR 方法对提取的 DNA 进行筛选和分型，以检测 EHV-1 和 EHV-4，然后进行测序和系统发育分析以确认病毒身份。选定的 EHV-1 和 EHV-4 阳性样本经马丹达比牛肾（MDBK）细胞系进行病毒分离：结果：结果显示，58/120（48%）份样本对 EHV 呈阳性。阳性样本的分型结果显示，在（48/120）40%的样本中检测到了 EHV-1，在（15/120）12%的样本中检测到了 EHV-4，而在 5 个样本中检测到了 EHV-1 和 4 的双重感染：结论：本次研究揭示了埃及马群中 EHV-1 和 EHV-4 持续循环的新数据，单匹马可能感染多种 EHV。此外，潜伏感染的马匹是病毒频繁再活化的潜在蓄水池。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.