Suppressed distribution of protein A on the surface of Staphylococcus aureus as a morphological characteristic of erythromycin-resistant strain

IF 1.2 4区医学 Q3 PATHOLOGY Medical Molecular Morphology Pub Date : 2024-02-22 DOI:10.1007/s00795-023-00379-4

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Abstract

To identify a new morphological phenotype of erythromycin (EM)-resistant Staphylococcus aureus (S. aureus) were isolated in vitro from EM-sensitive parent strain, and the distribution of staphylococcus specific protein A (SpA) on the surface of these strains was examined morphologically by using applied immunoelectron microscopy. The isolated EM-resistant strains had thickened cell walls, and the distribution of SpA on the surfaces of these strains was demonstrated to be lower than that of the parent strain. The SpA suppression was confirmed by enzyme-linked immunosorbent assay (ELISA) using fixed EM-resistant cells. Moreover, the spa gene of EM-resistant cells was detected by polymerase chain reaction (PCR) and confirmed by quantitative real-time PCR assay, showing that the expression of SpA was repressed at the transcriptional level in these strains. Furthermore, ELISA assay showed that whole EM-resistant cell SpA content was significantly decreased. Therefore, it was considered that the suppression of surface SpA on the EM-resistant strain was due to regulated SpA production, and not dependent on the conformational change in SpA molecule expression through cell wall thickening. These results strongly suggest that suppressed SpA distribution on the EM-resistant S. aureus is a phenotypical characteristic in these strains.

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金黄色葡萄球菌表面蛋白 A 的分布抑制是耐红霉素菌株的形态特征之一

摘要从体外分离出对红霉素（EM）敏感的金黄色葡萄球菌（S. aureus）亲本菌株，并用免疫电镜从形态学上观察了这些菌株表面葡萄球菌特异性蛋白 A（SpA）的分布。分离出的抗 EM 菌株细胞壁增厚，这些菌株表面的 SpA 分布低于亲本菌株。使用固定的 EM 抗性细胞进行酶联免疫吸附试验（ELISA）证实了 SpA 的抑制作用。此外，聚合酶链式反应（PCR）检测了 EM 抗性细胞的 spa 基因，并通过实时定量 PCR 检测进行了确认，结果表明这些菌株在转录水平上抑制了 SpA 的表达。此外，酶联免疫吸附试验（ELISA）表明，整个抗 EM 细胞中的 SpA 含量明显降低。因此，可以认为抗EM菌株表面SpA的抑制是由于SpA的产生受到了调控，而不是依赖于细胞壁增厚导致的SpA分子表达构象变化。这些结果有力地表明，抗 EM 金黄色葡萄球菌的 SpA 分布受抑制是这些菌株的表型特征。

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来源期刊

Medical Molecular Morphology 医学-病理学

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

>12 weeks

期刊介绍： Medical Molecular Morphology is an international forum for researchers in both basic and clinical medicine to present and discuss new research on the structural mechanisms and the processes of health and disease at the molecular level. The structures of molecules, organelles, cells, tissues, and organs determine their normal function. Disease is thus best understood in terms of structural changes in these different levels of biological organization, especially in molecules and molecular interactions as well as the cellular localization of chemical components. Medical Molecular Morphology welcomes articles on basic or clinical research in the fields of cell biology, molecular biology, and medical, veterinary, and dental sciences using techniques for structural research such as electron microscopy, confocal laser scanning microscopy, enzyme histochemistry, immunohistochemistry, radioautography, X-ray microanalysis, and in situ hybridization. Manuscripts submitted for publication must contain a statement to the effect that all human studies have been reviewed by the appropriate ethics committee and have therefore been performed in accordance with the ethical standards laid down in an appropriate version of the 1964 Declaration of Helsinki. It should also be stated clearly in the text that all persons gave their informed consent prior to their inclusion in the study. Details that might disclose the identity of the subjects under study should be omitted.