Exploring the modulation of MLH1 and MSH2 gene expression in hesperetin-treated breast cancer cells (BT-474).

IF 1.4 Q3 Pharmacology, Toxicology and Pharmaceutics Journal of Advanced Pharmaceutical Technology & Research Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI:10.4103/japtr.japtr_279_23
Ahmed Mohammed Salman, Esmaeil Babaei, Ahmed Salim Kadhim Al-Khafaji
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Abstract

The major mortality factor for women globally is breast cancer, and current treatments have several adverse effects. Hesperetin (HSP) is a flavone that occurs naturally with anti-tumor capabilities and has been investigated as a potential treatment for cancer. This study aimed to investigate the cytotoxic and anti-malignant potential of HSP on breast cancer cells (BT-474) and normal cells (MCF-10a). The results indicated that HSP has dose-dependent cytotoxicity in BT-474 and MCF-10a cells. The elevated concentration of HSP lowered cell viability and proliferation. The half-maximal inhibitory concentration (IC50) of HSP in BT-474 cancer cells after a 48-h exposure was 279.2 μM/ml, while the IC50 in normal cells was 855.4 μM/ml. The cytotoxicity of HSP was more significant in cancer cell lines than in normal cell lines and this aspect presents a favorable factor in utilizing the drug for the treatment of breast cancer. The apoptotic effect of HSP in BT-474 cells was investigated, and it was found that the higher the concentration of HSP more the cells underwent apoptosis. Furthermore, the highest concentration of HSP led to overexpression of the MLH1 and MSH2 genes in both breast cancer and normal cell lines. Overall, our study suggests that HSP has an anticancer effect on breast cancer cell lines, and the effect is concentration dependent.

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探索七叶皂苷(hesperetin)处理的乳腺癌细胞(BT-474)中 MLH1 和 MSH2 基因表达的调节。
全球妇女的主要死亡因素是乳腺癌,而目前的治疗方法有多种不良影响。橙皮素(Hesperetin,HSP)是一种天然黄酮,具有抗肿瘤能力,已被研究作为一种潜在的癌症治疗方法。本研究旨在探讨 HSP 对乳腺癌细胞(BT-474)和正常细胞(MCF-10a)的细胞毒性和抗恶性潜能。结果表明,HSP 对 BT-474 和 MCF-10a 细胞具有剂量依赖性细胞毒性。HSP 浓度升高会降低细胞活力和增殖。暴露 48 小时后,HSP 在 BT-474 癌细胞中的半最大抑制浓度(IC50)为 279.2 μM/ml,而在正常细胞中的 IC50 为 855.4 μM/ml。与正常细胞株相比,HSP 在癌细胞株中的细胞毒性更为显著,这为利用该药物治疗乳腺癌提供了有利条件。研究了 HSP 对 BT-474 细胞的凋亡效应,发现 HSP 浓度越高,细胞凋亡越多。此外,最高浓度的 HSP 会导致乳腺癌细胞株和正常细胞株中的 MLH1 和 MSH2 基因过度表达。总之,我们的研究表明,HSP 对乳腺癌细胞株有抗癌作用,而且这种作用与浓度有关。
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来源期刊
CiteScore
2.00
自引率
7.10%
发文量
44
审稿时长
20 weeks
期刊介绍: Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is an Official Publication of Society of Pharmaceutical Education & Research™. It is an international journal published Quarterly. Journal of Advanced Pharmaceutical Technology & Research (JAPTR) is available in online and print version. It is a peer reviewed journal aiming to communicate high quality original research work, reviews, short communications, case report, Ethics Forum, Education Forum and Letter to editor that contribute significantly to further the scientific knowledge related to the field of Pharmacy i.e. Pharmaceutics, Pharmacology, Pharmacognosy, Pharmaceutical Chemistry. Articles with timely interest and newer research concepts will be given more preference.
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