Novel finding of Paulownia fortunei endophytic rhizobia in China

Abstract

Previous research has mainly focused on breeding, timber supply, and physiological–biochemical characteristics of Paulownia fortunei. However, there has been limited attention given to its endophytic rhizobia and its potential benefits for plant growth due to a lack of knowledge about the existence of root nodules. In this study, we isolated nine bacterial strains from the root nodules after occasionally discovering the nodular-like structure in uncultivated three year P. fortunei roots and then sequenced the 16S rDNA and nifA sequencing to conduct the bacterial strain identifications. We then determined the carbon use capability and nitrogenase activity of the bacterial strains. The result of 16S rDNA sequencing indicated that the endophytic rhizobia belongs to the genus Agrobacterium, Rhizobium, Herbaspirillum and Burkholderia. The results of nifA sequencing indicated that the nifA gene was sequenced in strain PG-3, PG-5, PG-6, PG-7, PG-8 and PG-9 rather than in strain PG-1, PG-2 and PG-4. Besides, the nitrogenase activity of strain PG-9 was significantly higher than other bacterial strains. Redundancy analysis (RDA) results indicated that fructose, glucose, and sucrose were significantly related to the plant height, diameter, and biomass rather than other carbon sources and nitrogenase activity. Our research revealed that the majority of bacterial strains isolated from P. fortunei exhibited a broad carbon utilization pathway and have the potential to promote plant growth after re-Inoculation. This study aims to investigate the phylogeny of endophytic rhizobia in P. fortunei, and expand the range of non-legume hosts studied for future research on biological N-fixation.