An overview of point-of-care testing for infections in critically ill patients.

IF 3.9 3区 医学 Q1 PATHOLOGY Expert Review of Molecular Diagnostics Pub Date : 2024-03-01 Epub Date: 2024-02-27 DOI:10.1080/14737159.2024.2322146
Alena Moerman, Jan J De Waele, Jerina Boelens
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Abstract

Introduction: Molecular diagnostic systems for point-of-care (POC) testing are nowadays routinely used and are part of many labs. Although often intended for bedside use outside of the microbiology lab, there is still room for expansion.

Areas covered: This review discusses the two techniques that are currently the most widespread, real-time polymerase-chain reaction (RT-PCR) and loop-mediated isothermal amplification (LAMP). An overview is provided of the various manufacturers and products as well as the evidence and current use in clinical practice. The article further sheds light on some newer techniques, such as CRISPR-based diagnostics and lab-on-a-chip, which are still in development.

Expert opinion: With many new platforms and techniques still in the pipeline and their potential currently not yet fully exploited, we expect the use of molecular POC testing to increase in the years to come. However, even when used in hospital - in lab, the main advantages of the tests being fast and easy to perform already provide significant benefits in terms of patient outcome.

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重症患者感染的护理点检测概述。
导言:如今,用于护理点(POC)检测的分子诊断系统已成为许多实验室的常规设备。虽然该系统通常用于微生物实验室以外的床边使用,但仍有扩展的空间:本综述讨论了目前最普遍的两种技术:实时聚合酶链反应(RT-PCR)和环介导等温扩增(LAMP)。文章概述了各种制造商和产品以及证据和目前在临床实践中的使用情况。文章还进一步揭示了一些仍在开发中的新技术,如基于 CRISPR 的诊断技术和芯片实验室:专家观点:由于许多新平台和新技术仍在研发过程中,而且其潜力目前尚未得到充分挖掘,我们预计分子 POC 检测的使用在未来几年会越来越多。不过,即使是在医院实验室中使用,检测快速、简便的主要优势也能为患者带来显著的疗效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.60
自引率
0.00%
发文量
71
审稿时长
1 months
期刊介绍: Expert Review of Molecular Diagnostics (ISSN 1473-7159) publishes expert reviews of the latest advancements in the field of molecular diagnostics including the detection and monitoring of the molecular causes of disease that are being translated into groundbreaking diagnostic and prognostic technologies to be used in the clinical diagnostic setting. Each issue of Expert Review of Molecular Diagnostics contains leading reviews on current and emerging topics relating to molecular diagnostics, subject to a rigorous peer review process; editorials discussing contentious issues in the field; diagnostic profiles featuring independent, expert evaluations of diagnostic tests; meeting reports of recent molecular diagnostics conferences and key paper evaluations featuring assessments of significant, recently published articles from specialists in molecular diagnostic therapy. Expert Review of Molecular Diagnostics provides the forum for reporting the critical advances being made in this ever-expanding field, as well as the major challenges ahead in their clinical implementation. The journal delivers this information in concise, at-a-glance article formats: invaluable to a time-constrained community.
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