Corrigendum: The expression of MACC1 and its role in the proliferation and apoptosis of salivary adenoid cystic carcinoma

Abstract

Haifeng L, Liao X, Shen Z, Xiangfeng G, Haigang L, Huang Z. The expression of MACC1 and its role in the proliferation and apoptosis of salivary adenoid cystic carcinoma. J Oral Pathol Med. 2015; 44: 810-817.

The revision of our manuscript is prompted by the concerns raised by Jennifer Byrne and colleagues regarding the nucleotide sequence reagents used in our paper titled “The expression of MACC1 and its role in the proliferation and apoptosis of salivary adenoid cystic carcinoma.”

Upon thorough examination of the nucleotide sequence (5’-AAGAUUGGACUUGUACACUGCTT-3′) of the siRNA, we observed that the siRNA perfectly matches the mRNA of MACC1 after removing the overhanging TT sequence. It is worth noting that the overhanging TT, which does not correspond to the target sequence, is commonly incorporated in siRNA design to enhance thermodynamic stability and RNA interference activity (PMID: 25211666; PMID: 30660935). We speculate that the discrepancy in the sequences presented in the preprint by Jennifer Byrne and colleagues may be due to their algorithm not deleting the base sequence of “TT.”

Furthermore, the small interfering RNA (siRNA) of MACC1 used in our study was procured from GenePharma (Shanghai, China). The siRNA duplexes were synthesized and purified by GenePharma (Shanghai, China). Despite approximately 9 years having passed since the study, we have located the original purchase order. The nucleotide sequence of the siRNA for MACC1 is accurate. However, we identified an error in our manuscript where the antisense of the siRNA for MACC1 was mistakenly inverted. The correct antisense of the siRNA for MACC1 is as follows: 3’-TTUUCUAACCUGAACAUGUGACG-5′ or 5’-GCAGUGUACAAGUCCAAUCUUTT-3′.

We apologize for this error.