Searching for mechanisms driving root pressure in Zea mays—a transcriptomic approach

Abstract

While there are many theories and a variety of innovative datasets contributing to our understanding of the mechanism generating root pressure in vascular plants, we are still unable to produce a specific cellular mechanism for any species. To discover these mechanisms, we used RNA-Seq to explore differentially expressed genes in three different tissues between individual Zea mays plants expressing root pressure and those producing none. Working from the perspective that roots cells are utililizing a combination of osmotic exudation and hydraulic pressure mechanisms to generate positively-pressured flow of water into the xylem from the soil, we hypothesized that differential expression analysis would yield candidate genes coding for membrane transporters, ion channels, ATPases, and hormones with clear relevance to root pressure generation. In basal stem and coarse root tissue, we observed these classes of differentially expressed genes and more, including a strong cytoskeletal remodeling response. Fine roots displayed remarkably little differential expression relevant to root pressure, leading us to conclude that they either do not contribute to root pressure generation or are constitutively expressing root pressure mechanisms regardless of soil water content.