Comparison between a Tail Clamp and Electrical Stimulation for Sevoflurane Minimum Anesthetic Concentration Determination in Green Iguanas (Iguana iguana).

Laura R Ghussn, André A Justo, Mariana C Sanches, Silvia Rg Cortopassi, Adriano B Carregaro
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Abstract

This study aimed to compare the minimum anesthetic concentration (MAC) of sevoflurane in green iguanas using elec- trical stimulation and tail clamping as noxious stimuli. Seven adult green iguanas (Iguana iguana) weighing 580 to 1,300 g were enrolled. Each iguana was anesthetized twice after a 1-week washout period, with MAC being determined using a tail clamp (MACt) or electrical stimulation (MACe ) techniques. After sevoflurane mask induction and endotracheal intubation, the fraction of expired sevoflurane (Fe 'Sevo) was maintained at 3.1% for 15 min before noxious stimulation. In a bracketing design, the subsequent Fe'Sevo values were increased or decreased by 10% after positive or negative responses, respectively. Each targeted Fe 'Sevo was kept constant for 15 min before stimulation. In MACt, the noxious stimulus involved closing a Kelly hemostatic curved forceps to the first ratchet at the base of the tail. At the same site, in MACe, 2 30 × 0.8-mm hypodermic needles inserted 1 cm apart were connected to an electrical stimulator set to deliver 30 mA at 50 Hz at a 6.5-ms interval. The hemostat and the needles were repositioned 2 cm distally and on alternate tail sides at each stimulation round. Individual MAC was obtained when 2 consecutive crossover events occurred (a positive response preceding a negative response or vice versa), with the MAC of each group represented by the average of the individual MAC values. Median (interquartile range) values for the sevoflurane MAC did not differ significantly between groups (2.2 [2.2 to 2.8%] in MACe and 2.2 [1.8 to 3.5%] in MACt ; P = 0.812). Time to anesthesia induction, time to MAC measurement, heart rate (HR), end-tidal carbon dioxide (ET'CO2), and cloacal temperature were not different between groups. Both the tail-clamping and the electrical stimulation techniques yielded resembling sevoflurane MAC values in green iguanas, which makes the tail clamp a reliable alternative to electrical stimulation-based MAC research in this species.

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在测定绿鬣蜥(Iguana iguana)七氟烷最低麻醉浓度时尾部钳夹法与电刺激法的比较。
本研究旨在比较七氟醚在绿鬣蜥体内的最低麻醉浓度(MAC),采用电刺激和夹尾作为有害刺激。七只成年绿鬣蜥(Iguana iguana)体重在 580-1,300 克之间。每只绿鬣蜥在经过一周的冲洗期后都会被麻醉两次,并使用夹尾(MACt)或电刺激(MACe)技术测定MAC。在七氟烷面罩诱导和气管插管后,呼出七氟烷(Fe'Sevo)的比例在有害刺激前的 15 分钟内保持在 3.1%。在括号设计中,在出现阳性或阴性反应后,随后的 Fe'Sevo 值分别增加或减少 10%。每个目标 Fe'Sevo 值在刺激前 15 分钟保持不变。在 MACt 中,有害刺激包括将凯利止血弯钳夹在尾基部的第一个棘轮上。在 MACe 中的同一部位,将两根相距 1 厘米的 30 × 0.8 毫米皮下注射针连接到电刺激器上,设置为以 50 赫兹、6.5 毫秒间隔输出 30 毫安电流。在每轮刺激中,止血钳和针头在尾部两侧交替向远端移动 2 厘米。当发生 2 次连续交叉事件(阳性反应先于阴性反应或反之亦然)时,将获得单个 MAC 值,每组的 MAC 值由单个 MAC 值的平均值表示。七氟醚 MAC 中位值(四分位数间距)在各组间无显著差异(MACe 为 2.2 [2.2 至 2.8%],MACt 为 2.2 [1.8 至 3.5%];P = 0.812)。麻醉诱导时间、MAC测量时间、心率(HR)、潮气末二氧化碳(ET'CO₂)和泄殖腔温度在各组之间没有差异。在绿鬣蜥体内,夹尾技术和电刺激技术都能产生类似的七氟醚 MAC 值,因此在该物种中,夹尾技术是电刺激 MAC 研究的可靠替代方法。
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