Knockout of SlDCL2b attenuates the resistance of tomato to potato spindle tuber viroid infection.

IF 4.8 1区农林科学 Q1 PLANT SCIENCES Molecular plant pathology Pub Date : 2024-03-01 DOI:10.1111/mpp.13441

Yuhong Zhang, Xiaxia Tian, Huiyuan Xu, Binhui Zhan, Changyong Zhou, Shifang Li, Zhixiang Zhang

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Abstract

RNA interference, or RNA silencing, is an important defence mechanism against viroid infection in plants. Plants encode multiple DICER-LIKE (DCL) proteins that are key components of the RNA silencing pathway. However, the roles of different DCLs in defence responses against viroid infection remain unclear. Here, we determined the function of tomato DCL2b (SlDCL2b) in defence responses against potato spindle tuber viroid (PSTVd) infection using SlDCL2b loss-of-function tomato mutant plants. Compared with wild-type plants, mutant plants were more susceptible to PSTVd infection, developing more severe symptoms earlier and accumulating higher levels of PSTVd RNAs. Moreover, we verified the feedback mechanism for the regulation of SlDCL2b expression by miR6026. Functional blocking of tomato miR6026, by expressing its target mimics, can enhance resistance to PSTVd infection in tomato plants. These findings deepen the current understanding of RNAi-based resistance against viroid infection and provide a potentially new strategy for viroid control.

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敲除 SlDCL2b 可削弱番茄对马铃薯纺锤形块茎病毒感染的抗性。

RNA 干扰或 RNA 沉默是植物抵抗病毒感染的一种重要防御机制。植物编码多种 DICER-LIKE（DCL）蛋白，它们是 RNA 沉默途径的关键组成部分。然而，不同的 DCL 在抵御病毒感染中的作用仍不清楚。在这里，我们利用 SlDCL2b 功能缺失番茄突变体植株，确定了番茄 DCL2b（SlDCL2b）在马铃薯纺锤形块茎病毒（PSTVd）感染防御反应中的功能。与野生型植株相比，突变植株更易受 PSTVd 感染，更早地出现严重症状，并积累更高水平的 PSTVd RNA。此外，我们还验证了 miR6026 对 SlDCL2b 表达调控的反馈机制。通过表达番茄 miR6026 的靶标模拟物对其进行功能性阻断，可以增强番茄植株对 PSTVd 感染的抗性。这些发现加深了目前对基于 RNAi 的抗病毒病感染的理解，并为病毒病控制提供了一种潜在的新策略。

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来源期刊

Molecular plant pathology 生物-植物科学

CiteScore

9.40

自引率

4.10%

发文量

120

审稿时长

6-12 weeks

期刊介绍： Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.