Immediate administration of hTERT-MSCs-IDO1-EVs reduces hypoalbuminemia after spinal cord injury.

IF 0.8 4区 农林科学 Q3 ZOOLOGY Veterinary Research Forum Pub Date : 2024-01-01 Epub Date: 2024-01-15 DOI:10.30466/vrf.2023.2003942.3903
Shiva Amanollahi, Ahmad Reza Bahrami, Azadeh Haghighitalab, Hanieh Shaterzadeh Yazdi, Hossein Kazemi Mehrjerdi
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Abstract

Spinal cord injury (SCI) presents challenging and unpredictable neurological recovery. During inflammatory conditions, the amount of serum albumin and nutrition consumption decreases. Currently, it is proposed to measure serum albumin and glucose content in human or animal subjects to predict the recovery rate and the efficiency of treatments following SCI. In this study, the effect of extra-cellular vesicles (EVs) from immortalized human adipose tissue-derived mesenchymal stem cells (hTERT-MSCs) equipped with the ectopic expression of the human indoleamine 2,3-dioxygenase-1 (IDO1) gene on serum albumin and glucose levels was investigated. After pre-clearing steps of 72-hr conditioned media, small EVs (sEVs) were isolated based on the ultra-filtration method. They were encapsulated with a chitosan-based hydrogel. Five experimental groups (female rats, N = 30, ~ 230 g) were considered, including SCI, sham, hydrogel, control green fluorescent protein (GFP)-EVs and IDO1-EVs. The 60.00 µL of hydrogel or hydrogels containing 100 µg sEVs from GFP or IDO1-EVs were locally injected immediately after SCI (laminectomy of the T10 vertebra and clip compression). After 8 weeks, non-fasting serum glucose and albumin levels were measured. The results indicated that the level of serum albumin in the animals received IDO1-EVs (3.52 ± 0.04) was increased in comparison with the SCI group (3.00 ± 0.94). Also, these animals indicated higher glucose levels in their serum (250.17 ± 69.61) in comparison with SCI ones (214 ± 45.34). Although these changes were not statistically significant, they could be considered as evidence for the beneficial effects of IDO1-EVs administration in the context of SCI to reduce hypoalbuminemia and improve energy consumption. More detailed experiments are required to confirm these results.

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立即注射 hTERT-间充质干细胞-IDO1-EVs可降低脊髓损伤后的低白蛋白血症。
脊髓损伤(SCI)的神经功能恢复具有挑战性和不可预测性。在炎症条件下,血清白蛋白的含量和营养消耗会减少。目前,有人建议测量人或动物的血清白蛋白和葡萄糖含量,以预测脊髓损伤后的恢复速度和治疗效果。本研究调查了细胞外囊泡(EVs)对血清白蛋白和葡萄糖水平的影响,EVs来自异位表达人吲哚胺-2,3-二氧合酶-1(IDO1)基因的永生化人脂肪组织间充质干细胞(hTERT-MSCs)。经过 72 小时条件培养基的预清除步骤后,采用超滤法分离出小 EVs(sEVs)。它们被壳聚糖水凝胶包裹。实验分为五组(雌性大鼠,N = 30,体重约 230 克),包括 SCI 组、假组、水凝胶组、绿色荧光蛋白(GFP)-EVs 对照组和 IDO1-EVs 组。在 SCI(T10 椎板切除术和夹子压迫)后立即局部注射 60.00 µL 水凝胶或含有 100 µg sEVs 的 GFP 或 IDO1-EVs 水凝胶。8 周后,测量非空腹血清葡萄糖和白蛋白水平。结果表明,与 SCI 组(3.00 ± 0.94)相比,接受 IDO1-EVs 治疗的动物的血清白蛋白水平(3.52 ± 0.04)有所提高。此外,与 SCI 组(214 ± 45.34)相比,这些动物血清中的葡萄糖水平更高(250.17 ± 69.61)。尽管这些变化在统计学上并不显著,但它们可被视为在 SCI 情况下施用 IDO1-EVs 对降低低白蛋白血症和改善能量消耗有益的证据。要证实这些结果,还需要更详细的实验。
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来源期刊
Veterinary Research Forum
Veterinary Research Forum Veterinary-General Veterinary
CiteScore
1.50
自引率
0.00%
发文量
0
审稿时长
8 weeks
期刊介绍: Veterinary Research Forum (VRF) is a quarterly international journal committed to publish worldwide contributions on all aspects of veterinary science and medicine, including anatomy and histology, physiology and pharmacology, anatomic and clinical pathology, parasitology, microbiology, immunology and epidemiology, food hygiene, poultry science, fish and aquaculture, anesthesia and surgery, large and small animal internal medicine, large and small animal reproduction, biotechnology and diagnostic imaging of domestic, companion and farm animals.
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