The first genetic linkage map of sea buckthorn (Hippophae rhamnoides) and QTL mapping of growth-related traits

IF 2.4 3区农林科学 Q1 Agricultural and Biological Sciences Horticulture Environment and Biotechnology Pub Date : 2024-03-11 DOI:10.1007/s13580-024-00597-4

Ruiman Geng, Youzhi Han, Zhihong Liu, Qing Xie

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Abstract

Sea buckthorn (Hippophae rhamnoides), a hardy deciduous shrub of the family Elaeagnaceae, is known for its economic and ecological importance due to its wide use in medicine, food, windbreaks, and sand fixation. In this study, the first genetic linkage map of H. rhamnoides was constructed based on an F₁ population (H. rhamnoides subsp. mongolica ‘wulangemu’ × H. rhamnoides subsp. sinensis ‘wucixiong’) by using start codon targeted (SCoT), sequence-related amplified polymorphism (SRAP), and simple sequence repeat (SSR) markers. The linkage map contained 1143 markers with a total length of 1889.46 centimorgans (cM), including 12 linkage groups (LG) and covering 97.17% of the estimated genome, with an average marker density of 1.67 cM. According to the phenotypic variation analysis, 61 QTLs were identified. Among these QTLs, there were 18 QTLs for seedling height, 27 QTLs for ground diameter, two QTLs for primary branching angle, 11 QTLs for leaf width, and 3 QTLs for leaf shape index. The range of phenotypic variation explained by these QTLs was 10.7 ~ 21.9%. The QTLs identified by high-density linkage mapping reported in this study will be useful for the molecular breeding of sea buckthorn.

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沙棘（Hippophae rhamnoides）的首个遗传连锁图谱和生长相关性状的 QTL 图谱

沙棘（Hippophae rhamnoides）是一种坚韧的落叶灌木，属榆叶梅科，因其在医药、食品、防风固沙等方面的广泛应用而具有重要的经济和生态价值。本研究利用起始密码子定向标记（SCoT）、序列相关扩增多态性标记（SRAP）和简单序列重复标记（SSR），在 F1 群体（H. rhamnoides subsp.该连接图包含 1143 个标记，总长度为 1889.46 厘米（cM），包括 12 个连接组（LG），覆盖了估计基因组的 97.17%，平均标记密度为 1.67 cM。根据表型变异分析，确定了 61 个 QTL。其中，苗高 QTL 18 个，地径 QTL 27 个，主分枝角 QTL 2 个，叶宽 QTL 11 个，叶形指数 QTL 3 个。这些 QTL 解释的表型变异范围为 10.7% ~ 21.9%。本研究报告中通过高密度连接图谱鉴定的 QTLs 将有助于沙棘的分子育种。

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来源期刊

Horticulture Environment and Biotechnology Agricultural and Biological Sciences-Horticulture

CiteScore

4.30

自引率

4.20%

发文量

审稿时长

6 months

期刊介绍： Horticulture, Environment, and Biotechnology (HEB) is the official journal of the Korean Society for Horticultural Science, was launched in 1965 as the "Journal of Korean Society for Horticultural Science". HEB is an international journal, published in English, bimonthly on the last day of even number months, and indexed in Biosys Preview, SCIE, and CABI. The journal is devoted for the publication of original research papers and review articles related to vegetables, fruits, ornamental and herbal plants, and covers all aspects of physiology, molecular biology, biotechnology, protected cultivation, postharvest technology, and research in plants related to environment.