A histological examination of the effects of Ferula elaeochytris extract on kidney and liver tissues in myoglobinuric acute renal failure.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnic & Histochemistry Pub Date : 2024-04-01 Epub Date: 2024-03-14 DOI:10.1080/10520295.2024.2323973
Fatma Yıldız, Meltem Güngör, Perihan Sezginer, Tiince Aksak
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Abstract

Myoglobinuric acute renal failure (MARF) is a structural and functional disorder that occurs in the kidney following the release of muscle cell contents into the circulation. In this present study, possible protective and curative effects of Ferula elaeochytris extract against kidney and liver damage in experimentally induced MARF in a rat model were investigated. 3-4 Month-old, 200-250 g Sprague Dawley rats were divided into 8 equal groups with 7 rats per group. Group I was a no-intervention Control group. All groups except for the Group I were dehydrated for 16 hours. Following this dehydration, 50% v/v aqueous glycerol solution was injected into both hind leg muscles of the animals, at a dose of 8 ml/kg. The rats were given physiological saline (SF) once orally before the model was administered (Group II) and after the model was administered (Group V). Similarly, two different doses of Ferula elaeochytris root extract (40 mg/kg and 80 mg/kg) were dissolved in 2 ml of SF and administered orally before (Groups III and IV) and after (Group VI, VII) the model was created. Following the experimental period, kidney and liver tissues were removed from all groups, and fixed in 10% neutral formaldehyde solution for light microscopic examinations. Intracellular vacuolization, enlargement in the Bowman's space, widespread atrophy in the tubular structures, luminal enlargement, and desquamation were detected in the kidney tissue sections of all the experimental model groups. In the liver tissue sections, was detected hepatocyte degeneration, intracellular vacuolization, irregularity in cell membrane borders, and apoptotic bodies. These histopathological consequences of MARF were evaluated for all groups, and whereas a curative effect of Ferula elaeochytris could be seen, its protective effect was higher than its curative effect.

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阿魏提取物对肌红蛋白尿急性肾衰竭患者肾脏和肝脏组织影响的组织学研究。
肌红蛋白尿急性肾衰竭(MARF)是肌肉细胞内容物释放到血液循环中后肾脏出现的一种结构和功能紊乱。本研究调查了阿魏提取物对实验诱导的大鼠急性肾功能衰竭模型的肾脏和肝脏损伤可能具有的保护和治疗作用。将 3-4 个月大、体重 200-250 克的 Sprague Dawley 大鼠分为 8 组,每组 7 只。I 组为无干预对照组。除 I 组外,其他各组均脱水 16 小时。脱水后,以 8 毫升/千克的剂量向大鼠两条后腿肌肉注射 50%v/v 的甘油水溶液。在模型注射前(第二组)和模型注射后(第五组),分别给大鼠口服生理盐水(SF)一次。同样,将两种不同剂量的阿魏根提取物(40 毫克/千克和 80 毫克/千克)溶于 2 毫升生理盐水中,在建立模型前(第三组和第四组)和建立模型后(第六组和第七组)分别口服一次。实验结束后,取出所有组的肾脏和肝脏组织,用 10%的中性甲醛溶液固定,进行光学显微镜检查。在所有实验组的肾组织切片中均发现细胞内空泡化、鲍曼氏间隙增大、肾小管结构广泛萎缩、管腔扩大和脱屑。在肝脏组织切片中,检测到肝细胞变性、细胞内空泡化、细胞膜边界不规则和凋亡体。对所有实验组的 MARF 组织病理学后果进行了评估,虽然可以看出白花阿魏具有治疗作用,但其保护作用高于治疗作用。
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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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