Comparative Antibacterial Activity of Clay-Supported Silver Nanoparticles Prepared by Conventional Heating and Microwave Methods

Abstract

The synthesis of multifunctional composites still relies on the use of conventional methods. However, these methods are expensive, time consuming and require high volumes of reducing agents which are often toxic. In this study, composites of bentonite-supported silver nanoparticles were prepared comparatively by the conventional heating method and the rapid microwave method; and their antibacterial activity was investigated against Escherichia coli and Staphylococcus aureus. The crystalline nature of the composites was evaluated by X-ray diffraction (XRD), while transmission electron microscope (TEM) coupled with energy-dispersive spectroscope was used for morphology and elemental analysis, respectively. Surface area and pore size analysis of the composites were conducted by the Brunauer, Emmett and Teller analyzer. TEM images revealed successful synthesis of the composites with a better dispersion of the nanoparticles achieved through microwave, where nanoparticle sizes were 6–38 nm and 9–56 nm by the conventional method. It is worth noting that the composites were prepared in less than 30 min using microwave as compared to 2 h of the conventional method. The XRD spectra confirmed the formation of silver and not any other impurities of the metal. These results revealed that, although the two methods are comparable, microwave method is efficient and time saving and can, therefore, synthesize composites with well-dispersed and narrow distributed nanoparticles. The antibacterial results demonstrated that the prepared composites are effective in the inactivation of various bacteria. These composites could be applied in water treatment, wound dressing, packaging, etc.