Evaluation of Aortic Hemodynamics Using Four-Dimensional Flow of Magnetic Resonance Imaging in Rabbits with Liver Fibrosis

Abstract

Background

Liver fibrosis (LF) precipitates systemic hemodynamic alterations, however, its impact on the aorta remaining undefined.

Purpose

To assess aorta hemodynamics changes during LF development in a rabbit model.

Study Type

Prospective, experimental.

Animal Model

Thirty 7-month-old male rabbits underwent bile duct ligation (BDL) to induce LF.

Field Strength/Sequence

Biweekly four-dimensional (4D) flow imaging incorporating a 3D gradient-echo at 3.0 T scanner for 14 weeks post-BDL.

Assessment

Histopathological exams for 2–5 rabbits were performed at each time point, following each MRI scan. LF was graded using the Metavir scale by a pathologist. 4D flow was analyzed by two radiologists using dedicated postprocessing software. They recorded 4D flow parameters at four aorta sections (aortic sinus, before and after bifurcation of aortic arch, and descending aorta).

Statistical Tests

The linear mixed model; Bonferroni correction; Pearson correlation coefficient (r); receiver operating characteristic (ROC) curve; Delong test. The level of significance was set at P < 0.05.

Results

Following BDL, the wall shear stress (WSS) (0.23–0.32 Pa), energy loss (EL) (0.27–1.55 mW) of aorta significantly increased at the second week for each plane, peaking at the sixth week (WSS: 0.35–0.49 Pa, EL: 0.57–2.0 mW). So did the relative pressure difference (RPD) (second week: 1.67 ± 1.63 mmHg, sixth week: 2.43 ± 0.63 mmHg) in plane 2. Notably, the RPD in plane 2 at the second week displayed the highest area under ROC curve of 0.998 (specificity: 1, sensitivity: 0.967). LF were found at the second, fourth, and sixth week after BDL, with grade F2, F3, and F4, respectively. The RPD in plane 2 was most strongly correlated with the severity of LF (r = 0.86).

Data Conclusions

The occurrence of LF could increase WSS, EL, and RPD of aorta as early as the second week following BDL.

Level of Evidence

1

Technical Efficacy

Stage 2