The kinetics of goat sperm is negatively affected after freezing in an extender including zinc oxide nanoparticles.

IF 1 4区生物学 Q3 BIOLOGY Cryo letters Pub Date : 2024-03-01

L C Pereira Arruda, G de Oliveira Alves Pinto, G F Carneiro, M M Pessoa Guerra

{"title":"The kinetics of goat sperm is negatively affected after freezing in an extender including zinc oxide nanoparticles.","authors":"L C Pereira Arruda, G de Oliveira Alves Pinto, G F Carneiro, M M Pessoa Guerra","doi":"","DOIUrl":null,"url":null,"abstract":"Background: Nanotechnology can benefit livestock industries, especially through postharvest semen manipulation. Zinc oxide nanoparticles (Np-ZnO) are potentially an example.Objective: To investigate how the addition of zinc oxide nanoparticles (Np-ZnO) affected the characteristics of post-thawed goat semen.Materials and methods: Seminal pools from four Saanen bucks were used. Semen was diluted in Tris-egg yolk extender, supplemented with Np-ZnO (0, 50, 100 or 200 ug/mL), frozen and stored in liquid nitrogen (-196 degree C), and thawed in a water bath (37 degree C / 30 s). Semen samples were evaluated for sperm kinetics by computer-assisted sperm analysis (CASA), and assessed for other functional properties by epifluorescence microscopy, such as plasma membrane integrity (PMi), acrosomal membrane integrity (ACi) and mitochondrial membrane potential (MMP).Results: For total motility (TM), the group treated with 200 ug/mL Np-ZnO was superior to the control. In straight-line velocity (VSL), the control was better than the group containing 200 ug/mL of Np-ZnO. For average path velocity (VAP), the control was higher than with 100 ug/mL Np-ZnO. For linearity (LIN), the control was higher than with 200 μg/mL Np-ZnO. In straightness (STR), the control and 100 μg/mL Np-ZnO were higher than with 200 ug/mL Np-ZnO. In wobble (WOB), the control was better than the 50 μg/mL Np-ZnO treatment. In PMi, ACi and MMP no significant differences were found.Conclusion: The addition of Np-ZnO (200 ug/mL) to the goat semen freezing extender improved the total motility of cells, whilst negatively affecting sperm kinetics. https://doi.org/10.54680/fr24210110512.","PeriodicalId":10937,"journal":{"name":"Cryo letters","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cryo letters","FirstCategoryId":"99","ListUrlMain":"","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Nanotechnology can benefit livestock industries, especially through postharvest semen manipulation. Zinc oxide nanoparticles (Np-ZnO) are potentially an example.

Objective: To investigate how the addition of zinc oxide nanoparticles (Np-ZnO) affected the characteristics of post-thawed goat semen.

Materials and methods: Seminal pools from four Saanen bucks were used. Semen was diluted in Tris-egg yolk extender, supplemented with Np-ZnO (0, 50, 100 or 200 ug/mL), frozen and stored in liquid nitrogen (-196 degree C), and thawed in a water bath (37 degree C / 30 s). Semen samples were evaluated for sperm kinetics by computer-assisted sperm analysis (CASA), and assessed for other functional properties by epifluorescence microscopy, such as plasma membrane integrity (PMi), acrosomal membrane integrity (ACi) and mitochondrial membrane potential (MMP).

Results: For total motility (TM), the group treated with 200 ug/mL Np-ZnO was superior to the control. In straight-line velocity (VSL), the control was better than the group containing 200 ug/mL of Np-ZnO. For average path velocity (VAP), the control was higher than with 100 ug/mL Np-ZnO. For linearity (LIN), the control was higher than with 200 μg/mL Np-ZnO. In straightness (STR), the control and 100 μg/mL Np-ZnO were higher than with 200 ug/mL Np-ZnO. In wobble (WOB), the control was better than the 50 μg/mL Np-ZnO treatment. In PMi, ACi and MMP no significant differences were found.

Conclusion: The addition of Np-ZnO (200 ug/mL) to the goat semen freezing extender improved the total motility of cells, whilst negatively affecting sperm kinetics. https://doi.org/10.54680/fr24210110512.

微信好友朋友圈 QQ好友复制链接

本刊更多论文

山羊精子在包括纳米氧化锌颗粒在内的扩展剂中冷冻后，其动力学会受到负面影响。

背景：纳米技术可造福畜牧业，特别是通过采精后的处理。纳米氧化锌（Np-ZnO）就是一个潜在的例子：研究添加纳米氧化锌（Np-ZnO）如何影响解冻后山羊精液的特性：材料：使用四只萨能公羊的精液池。精液用三聚氰胺蛋黄扩展剂稀释，添加 Np-ZnO（0、50、100 或 200 微克/毫升），冷冻并储存在液氮中（-196 摄氏度），然后在水浴中解冻（37 摄氏度/30 秒）。精液样本通过计算机辅助精子分析（CASA）评估精子动力学，并通过外荧光显微镜评估其他功能特性，如质膜完整性（PMi）、顶体膜完整性（ACi）和线粒体膜电位（MMP）：在总运动能力（TM）方面，用 200 微克/毫升 Np-ZnO 处理的组优于对照组。在直线速度（VSL）方面，对照组优于含有 200 微克/毫升 Np-ZnO 的组。在平均路径速度（VAP）方面，对照组高于含有 100 微克/毫升 Np-ZnO 的组别。在线性度（LIN）方面，对照组高于含有 200 微克/毫升 Np-ZnO 的组。在直线度（STR）方面，对照组和 100 μg/mL Np-ZnO 均高于 200 微克/毫升 Np-ZnO。在摇摆（WOB）方面，对照组优于 50 微克/毫升 Np-ZnO 处理组。在 PMi、ACi 和 MMP 中没有发现明显的差异：https://doi.org/10.54680/fr24210110512。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Cryo letters 生物-生理学

CiteScore

1.80

自引率

10.00%

发文量

审稿时长

1 months

期刊介绍： A bimonthly international journal for low temperature sciences, including cryobiology, cryopreservation or vitrification of cells and tissues, chemical and physical aspects of freezing and drying, and studies involving ecology of cold environments, and cold adaptation The journal publishes original research reports, authoritative reviews, technical developments and commissioned book reviews of studies of the effects produced by low temperatures on a wide variety of scientific and technical processes, or those involving low temperature techniques in the investigation of physical, chemical, biological and ecological problems.