Alizarin Red S as a Chromogenic Agent for the Determination of Meropenem in Pharmaceutical- Formulations

Abstract

Alizarin red S (ALRS)reagent has been used for to determination of meropenem (MEP)by an easy, sensitive, and selective procedure based on an ion-pair formation reaction between ALRS and MEP in an aqueous: alcoholic medium in the ratio of 50:50 without adjustment of the acidity of the reaction medium. The linearity ranged from 1 to 50 µg /mL, molar absorptivity was 6.557 × 10 3 L.mol -1 .cm -1 ., index of Sandell’s, was 585584 µg.cm -2 , and the calculated LOD and LOQ were 0.02789 µg/mL, 0.0929 µg/mL respectively. The calculated range of relative error was from -1.022 to 0.34 % which indicates high accuracy, and the calculated range of the relative standard deviation was from 0.347 to 0.574% which indicates high precision. The method has been applied for the determination of MEP in dosage forms successfully in which the recovery ranged from 99.8 to 103.2%. The standard addition method proves no interfering effect caused by inactive ingredients involved in dosage forms of meropenem