Fenugreek galactomannan: High-Performance Thin-Layer Chromatography (HPTLC) method for identification and quantification of galactose and mannose

Pharmacia Pub Date : 2024-02-23 DOI:10.3897/pharmacia.71.e118029

Vanya Nalbantova, Velislava Todorova, N. Benbassat, Cedric Delattre

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Abstract

Fenugreek (Trigonella foenum-graecum L.) is a leguminous, medicinal plant that finds applications in traditional medicine and as an agent in pharmaceutical and nutraceutical products. Its seeds contain various compounds to which numerous beneficial effects are attributed, such as soluble polysaccharides, in particular galactomannan. There are limited data reporting the determination of Bulgarian fenugreek galactomannan composition by HPTLC. The analysis was successful in separating galactose and mannose, each demonstrating different Rf values 0.36, 0.48, respectively. After developing the method with the appropriate conditions, it was validated for linearity, accuracy and precision, range, limits of detection (LD) and quantification (LQ), and robustness. The LD of galactose and mannose was found at 91.34 ng/band and 85.26 ng/band, respectively. The method confirmed the composition of hydrolyzed galactomannan from fenugreek, revealing a ratio of galactose to mannose closed to 1:1, and this capability can be of significant value in both the pharmaceutical and food industries.

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葫芦巴半乳甘露聚糖：鉴定和定量半乳糖和甘露糖的高效薄层色谱法 (HPTLC)

胡芦巴（Trigonella foenum-graecum L.）是一种豆科药用植物，可用于传统医药，也可作为药物和保健品的制剂。它的种子含有多种化合物，如可溶性多糖，特别是半乳甘露聚糖，具有多种有益功效。通过 HPTLC 测定保加利亚葫芦巴半乳甘露聚糖成分的数据有限。这项分析成功地分离了半乳糖和甘露糖，它们分别显示出不同的 Rf 值 0.36 和 0.48。在适当的条件下建立方法后，对其线性、准确度和精密度、范围、检出限（LD）和定量限（LQ）以及稳健性进行了验证。半乳糖和甘露糖的检出限分别为 91.34 纳克/带和 85.26 纳克/带。该方法证实了从葫芦巴中提取的水解半乳甘露聚糖的组成，显示半乳糖和甘露糖的比例接近 1:1，这种能力在制药和食品行业都具有重要价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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