In vitro Evaluation of Anticancer Activity of Karanthai Legium (Kl) – A Siddha Medicine for Cervical Carcinoma against Hela Cell Line By MTT Assay

Abstract

Cervical cancer become a major global burden and of course considered one of the leading causes of cancer-related deaths in women, particularly in developing countries. Viral induction of human papilloma viruses (HPV) can disturb the basic cellular mechanism of growth control. Currently medicinal plants have become the source of drug discovery in research for treating cancer. Siddha medicine is an ancient medical system of India which classifies diseases into 4448 types. In Siddha literature, Cancer is mentioned as Vippuruthinoipadalam (Carcinoma like illness), Kiranthi noi, Mega Katti, Pilavai and Putru noi (tumour). Literary evidences have a classic collection of herbal, mineral, herbo- mineral medicines for Cancer. Many anti-cancerous drugs are now in practice in Siddha medicine. Among them, Karanthai Legium (KL) a classical herbo mineral drug having anticancer potential is mentioned in Siddha Literature. The main objective of this study is to evaluate anti- cancer activity against HeLa cell using MTT assay. Present study revealed that, Karanthailegium (KL) at high as 200µg/ml showed high cytotoxicity activity as high as 72.44% against HeLa cell lines. IC50 of the tested KL against HeLa cells was calculated as104.90µg/ml. It was concluded from the result of the present study that the formulation KL possesses promising anti-cancer activity.