Optical Coherence Tomography (OCT) Alterations Choice for Aquatic Organism Anatomy and Morphology Analysis: A Case Study of A Giant River Prawn (Macrobrachium Rosenbergii)

Abstract

In this study, a Frequency Domain Optical Coherence Tomography (FD-OCT) system was utilized for non-invasive imaging of the giant river prawn (Macrobrachium rosenbergii) to analyze its anatomy and physiology. Seven tissue parts were examined: both stalked eyes (compound eyes), both sides of the carapace, the middle and lower ventral abdominal segments, and the uropod. The results indicated that FD-OCT was effective in detecting all the tissues studied. The deepest area detected was the eyestalks, with a range of approximately 930-1000 μm, while the shallowest detected areas were the ventral and dorsal segments, ranging from approximately 0-100 μm to 300-500 μm. This variance in detection range may be attributed to the dense exoskeleton and muscle bundles, which result in lower penetration in the ventral and dorsal segments compared to the eyestalks. Overall, this study demonstrated that FD-OCT can provide valuable insights into the tissue structure of giant river prawns and other crustaceans, offering significant benefits for further anatomical and physiological research.