First report Calonectria hawksworthii as an aetiological agent of fruit rot of cocoa in Brazil

IF 1.1 4区农林科学 Q3 PLANT SCIENCES Journal of Phytopathology Pub Date : 2024-04-13 DOI:10.1111/jph.13305

Maruzanete Pereira de Melo, Sara de Sousa Martins, Poliana Prates de Souza Soares, Naasoom Luiz Santos Mesquita, Armínio Santos, Quelmo Silva de Novaes, Vitória Cristina Santiago Alves, José Luiz Bezerra, Jadson Diogo Pereira Bezerra

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Abstract

Cacao (Theobroma cacoa L.) is an important tropical fruit tree used in chocolate production. Among the biotic problems interfering with fruit production, phytopathogenic fungi stand out. During a visit to cocoa production areas in the municipality of Medicilândia, Pará, Brazil (Amazon region), we observed cocoa fruits with dark lesions and greyish-white cottony mycelia growth. In order to determine the aetiological agent's identity, we extracted DNA and amplified part of the TEF-1α and RBP2 genes and compared them with sequences in GenBank using the BLASTn tool. Based on molecular results and phylogenetic analysis, the isolates were identified as Calonectria hawksworthii. Inoculations in cacao fruits showed symptoms after 6 days and were similar to those collected in the field, confirming the postulates. This study is the first report of Ca. hawksworthii causing cocoa bean rot worldwide.

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首次报告 Calonectria hawksworthii 是巴西可可果腐病的病原体

可可（Theobroma cacoa L.）是一种重要的热带果树，用于生产巧克力。在干扰果实生产的生物问题中，植物病原真菌最为突出。在对巴西帕拉州美迪西兰迪亚市（亚马逊地区）的可可生产区进行考察期间，我们观察到可可果实出现黑色病变，并长出灰白色棉状菌丝。为了确定病原体的身份，我们提取了 DNA 并扩增了部分 TEF-1α 和 RBP2 基因，然后使用 BLASTn 工具将其与 GenBank 中的序列进行比较。根据分子结果和系统进化分析，确定分离物为 Calonectria hawksworthii。可可果实接种 6 天后出现症状，与田间采集的症状相似，证实了上述推测。这项研究是全球首次报道 Ca. hawksworthii 引起可可豆腐烂病。

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来源期刊

Journal of Phytopathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays. Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes. Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.